A causal multiomics study discriminates the early immune features of Ad5-vectored Ebola vaccine recipients

Zhe Zhang; Wenjing Yang; Zhengshan Chen; Haoang Chi; Shipo Wu; Wanru Zheng; Ruochun Jin; Busen Wang; Yudong Wang; Nan Huo; Jinlong Zhang; Xiaohong Song; Liyang Xu; Jun Zhang; Lihua Hou; Wei Chen

doi:10.1016/j.xinn.2024.100603

A causal multiomics study discriminates the early immune features of Ad5-vectored Ebola vaccine recipients

Innovation (Camb). 2024 Mar 2;5(3):100603. doi: 10.1016/j.xinn.2024.100603. eCollection 2024 May 6.

Authors

Zhe Zhang¹, Wenjing Yang², Zhengshan Chen¹, Haoang Chi^{3

2}, Shipo Wu¹, Wanru Zheng¹, Ruochun Jin², Busen Wang¹, Yudong Wang¹, Nan Huo¹, Jinlong Zhang¹, Xiaohong Song¹, Liyang Xu², Jun Zhang¹, Lihua Hou¹, Wei Chen¹

Affiliations

¹ Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
² Department of Intelligent Data Science, College of Computer Science and Technology, National University of Defense Technology, Changsha 410073, China.
³ Intelligent Game and Decision Lab, Academy of Military Science, Beijing 100091, China.

Abstract

The vaccine-induced innate immune response is essential for the generation of an antibody response. To date, how Ad5-vectored vaccines are influenced by preexisting anti-Ad5 antibodies during activation of the early immune response remains unclear. Here, we investigated the specific alterations in GP_1,2-specific IgG-related elements of the early immune response at the genetic, molecular, and cellular levels on days 0, 1, 3, and 7 after Ad5-EBOV vaccination. In a causal multiomics analysis, distinct early immune responses associated with GP_1,2-specific IgG were observed in Ad5-EBOV recipients with a low level of preexisting anti-Ad5 antibodies. This study revealed the correlates of the Ad5-EBOV-induced IgG response and provided mechanistic evidence for overcoming preexisting Ad5 immunity during the administration of Ad5-vectored vaccines.