Immobilization of a broad host range phage on the peroxidase-like Fe-MOF for colorimetric determination of multiple Salmonella enterica strains in food

Mikrochim Acta. 2024 May 15;191(6):331. doi: 10.1007/s00604-024-06402-4.

Abstract

A broad host range phage-based nanozyme (Fe-MOF@SalmpYZU47) was prepared for colorimetric detection of multiple Salmonella enterica strains. The isolation of a broad host range phage (SalmpYZU47) capable of infecting multiple S. enterica strains was achieved. Then, it was directly immobilized onto the Fe-MOF to prepare Fe-MOF@SalmpYZU47, exhibiting peroxidase-like activity. The peroxidase-like activity can be specifically inhibited by multiple S. enterica strains, benefiting from the broad host range capture ability of Fe-MOF@SalmpYZU47. Based on it, a colorimetric detection approach was developed for S. enterica in the range from 1.0 × 102 to 1.0 × 108 CFU mL-1, achieving a low limit of detection (LOD) of 11 CFU mL-1. The Fe-MOF@SalmpYZU47 was utilized for detecting S. enterica in authentic food samples, achieving recoveries ranging from 91.88 to 105.34%. Hence, our proposed broad host range phage-based nanozyme exhibits significant potential for application in the colorimetric detection of pathogenic bacteria.

Keywords: Salmonella enterica; Colorimetric determination; Fe-MOF nanozyme; Food samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colorimetry* / methods
  • Food Contamination / analysis
  • Food Microbiology / methods
  • Limit of Detection*
  • Metal-Organic Frameworks* / chemistry
  • Peroxidase / chemistry
  • Salmonella enterica* / chemistry
  • Salmonella enterica* / isolation & purification