Bead-beating assay during synovial fluid DNA extraction improves real-time PCR accuracy for periprosthetic joint infection

Yuta Hieda; Hyonmin Choe; Hiroyuki Ike; Koki Abe; Ken Kumagai; Masanobu Takeyama; Yusuke Kawabata; Naomi Kobayashi; Yutaka Inaba

doi:10.1002/jor.25871

Bead-beating assay during synovial fluid DNA extraction improves real-time PCR accuracy for periprosthetic joint infection

J Orthop Res. 2024 May 13. doi: 10.1002/jor.25871. Online ahead of print.

Authors

Yuta Hieda¹, Hyonmin Choe¹, Hiroyuki Ike¹, Koki Abe¹, Ken Kumagai¹, Masanobu Takeyama¹, Yusuke Kawabata¹, Naomi Kobayashi², Yutaka Inaba¹

Affiliations

¹ Department of Orthopaedic Surgery, Yokohama City University, Yokohama City, Kanagawa, Japan.
² Department of Orthopaedic Surgery, Yokohama City University Medical Center, Yokohama City, Kanagawa, Japan.

PMID: 38741241
DOI: 10.1002/jor.25871

Abstract

Polymerase chain reaction (PCR)-based genetic diagnosis is a rapid and sensitive method to diagnose periprosthetic joint infection (PJI). DNA extraction using bead beating is an effective method for collecting bacterial genes in Gram-positive bacteria. We compared the detection accuracy between the conventional and bead-beating DNA extraction assay. The detection rate improved from 86.7% using the conventional method to 95.6% using the bead-beating. Our results suggest that bead-beating during DNA extraction can improve the accuracy of PCR-based genetic diagnosis of PJI.

Publication types

Editorial