Automated radiosynthesis of [11C]CPPC for in-human PET imaging applications

Huailei Jiang; Pritam Roy; Yan Guo; Otto Muzik; Eric A Woodcock

doi:10.62347/MXKZ6739

Automated radiosynthesis of [¹¹C]CPPC for in-human PET imaging applications

Am J Nucl Med Mol Imaging. 2024 Apr 25;14(2):144-148. doi: 10.62347/MXKZ6739. eCollection 2024.

Authors

Huailei Jiang^{1

2

3}, Pritam Roy^{1

2

3}, Yan Guo^{1

2

3}, Otto Muzik^{2

4}, Eric A Woodcock^{5

6}

Affiliations

¹ Cyclotron and Radiochemistry Core, Karmanos Cancer Institute Detroit, MI, USA.
² PET Center, Karmanos Cancer Institute Detroit, MI, USA.
³ Department of Oncology, Wayne State University Detroit, MI, USA.
⁴ Department of Pediatrics and Neurology, Wayne State University Detroit, MI, USA.
⁵ Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine Detroit, MI, USA.
⁶ Department of Pharmacology, Wayne State University School of Medicine Detroit, MI, USA.

Abstract

The macrophage colony-stimulating factor 1 receptor (CSF1R) is almost exclusively expressed in microglia, representing a biomarker target for imaging of microglia availability. [¹¹C]CPPC has specific binding affinity to CSF1R and suitable kinetic properties for in vivo PET imaging of microglia. However, previous studies reported a low radiochemical yield, motivating additional research to optimize [¹¹C]CPPC radiochemistry. In this work, we report an automated radiosynthesis of [¹¹C]CPPC on a Synthra MeIPlus module with improved radiochemical yield. The final [¹¹C]CPPC product was obtained with excellent chemical/radiochemical purities and molecular activity, facilitating high-quality in-human PET imaging applications.

Keywords: PET imaging; [11C]CPPC; automation; radiopharmaceutical; radiosynthesis.