A reproducible and affordable method of conducting luciferase assay

Kirtana Reddy; Balaji Ramachandran

doi:10.1016/j.ab.2024.115558

A reproducible and affordable method of conducting luciferase assay

Anal Biochem. 2024 May 10:692:115558. doi: 10.1016/j.ab.2024.115558. Online ahead of print.

Authors

Kirtana Reddy¹, Balaji Ramachandran²

Affiliations

¹ Department of Molecular Oncology, Cancer Institute WIA, Adyar, Chennai, 600 036, India.
² Department of Molecular Oncology, Cancer Institute WIA, Adyar, Chennai, 600 036, India. Electronic address: balajiphd@gmail.com.

PMID: 38735426
DOI: 10.1016/j.ab.2024.115558

Abstract

Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.

Keywords: Dual-Luciferase assay; Firefly luciferase assay buffer; Luciferin and coelenterazine; Passive lysis buffer; Renilla luciferase assay buffer.