HIF-1α facilitates glioma proliferation and invasion by activating pyroptosis signaling axis

Xin-Wei Wang; Hao Fu; Ya-Min Zhang

doi:10.1186/s41016-024-00366-3

HIF-1α facilitates glioma proliferation and invasion by activating pyroptosis signaling axis

Chin Neurosurg J. 2024 May 11;10(1):14. doi: 10.1186/s41016-024-00366-3.

Authors

Xin-Wei Wang¹, Hao Fu², Ya-Min Zhang³

Affiliations

¹ The First Central Clinical School, Tianjin Medical University, Tianjin, 300142, China.
² Department of General Medicine, Characteristic Medical Center of PAP, Tianjin, 300162, China.
³ The First Central Clinical School, Tianjin Medical University, Tianjin, 300142, China. 5020200824@nankai.edu.cn.

Abstract

Background: HIF-1α is thought to be a novel regulator which contributes to carcinogenesis. However, the mechanism underlying the effect of HIF-1α in gliomas remains largely unknown.

Methods: In the research, we demonstrate that HIF-lα mRNA and protein levels are elevated in glioma cells. The colony formation assays, transwell assays, and wound-healing assays showed that overexpression of HIF-1α promoted proliferation and invasion of glioma cells.

Results: Overexpression of HIF-lα also increased the expression of inflammatory factors related to pyrolysis (TNF-α, IL-10, and IL-1β) and protein related to pyrolysis signal pathway (NLRP3, ASC, caspase-1, GSDMD, and GSDME).

Conclusions: Therefore, we speculate that HIF-1α promotes the proliferation and invasion of glial cells by regulating pyrolysis pathway. These results might provide a novel strategy and target for treatment of glioma.

Keywords: Glial cells; Hypoxia-inducing factor 1α; Pyroptosis; SHG44 cells.

Grants and funding

CWJ18L004/Military Research Project