A taste of a toxin paradise: Xenorhabdus and Photorhabdus bacterial secondary metabolites against Aedes aegypti larvae and eggs

Derya Ulug; Mustapha Touray; Sebnem Hazal Gulsen; Harun Cimen; Canan Hazir; Helge B Bode; Selcuk Hazir

doi:10.1016/j.jip.2024.108126

A taste of a toxin paradise: Xenorhabdus and Photorhabdus bacterial secondary metabolites against Aedes aegypti larvae and eggs

J Invertebr Pathol. 2024 May 10:205:108126. doi: 10.1016/j.jip.2024.108126. Online ahead of print.

Authors

Derya Ulug¹, Mustapha Touray², Sebnem Hazal Gulsen³, Harun Cimen⁴, Canan Hazir⁵, Helge B Bode⁶, Selcuk Hazir²

Affiliations

¹ Department of Biology, Faculty of Science, Aydin Adnan Menderes University, 09100, Aydin, Türkiye. Electronic address: deryaasici@adu.edu.tr.
² Department of Biology, Faculty of Science, Aydin Adnan Menderes University, 09100, Aydin, Türkiye.
³ Department of Plant and Animal Production, Kocarli Vocational School, Aydin Adnan Menderes University, 09100 Aydin, Türkiye.
⁴ Recombinant DNA and Recombinant Protein Application and Research Center, Aydin Adnan Menderes University, 09100, Aydin, Türkiye.
⁵ Aydin Health Services Vocational School, Adnan Menderes University, 09100 Aydin, Türkiye.
⁶ Max-Planck-Institute for Terrestrial Microbiology, Department of Natural Products in Organismic Interactions, 35043 Marburg, Germany; Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany; Center for Synthetic Microbiology, Phillips University Marburg, 35043 Marburg, Germany; Department of Chemistry, Phillips University Marburg, 35043 Marburg, Germany; Senckenberg Gesellschaft für Naturforschung, Frankfurt am Main, 60325, Germany.

PMID: 38734162
DOI: 10.1016/j.jip.2024.108126

Abstract

Aedes-transmitted arboviral infections such as Dengue, Yellow Fever, Zika and Chikungunya are increasing public health problems. Xenorhabdus and Photorhabdus bacteria are promising sources of effective compounds with important biological activities. This study investigated the effects of cell-free supernatants of X. szentirmaii, X. cabanillasii and P. kayaii against Ae. aegypti eggs and larvae and identified the bioactive larvicidal compound in X. szentirmaii using The EasyPACId method. Among the three tested bacterial species, X. cabanillasii exhibited the highest (96%) egg hatching inhibition and larvicidal activity (100% mortality), whereas P. kayaii was the least effective species in our study. EasyPACId method revealed that bioactive larvicidal compound in the bacterial supernatant was fabclavine. Fabclavines obtained from promoter exchange mutants of different bacterial species such as X. cabanillasii, X. budapestensis, X. indica, X. szentirmaii, X. hominckii and X. stockiae were effective against mosquito larvae. Results show that these bacterial metabolites have potential to be used in integrated pest management (IPM) programmes of mosquitoes.

Keywords: Biological control; EasyPACId method; Fabclavine; Larvicidal; Natural products; Ovicidal.