Comparison of passive targeted delivery of inorganic and organic nanocarriers among different types of tumors

Alisa S Postovalova; Yulia A Tishchenko; Maria S Istomina; Timofey E Karpov; Sergei A Shipilovskikh; Daria Akhmetova; Anna Rogova; Nina V Gavrilova; Alexander S Timin

doi:10.1016/j.nano.2024.102753

Comparison of passive targeted delivery of inorganic and organic nanocarriers among different types of tumors

Nanomedicine. 2024 May 9:59:102753. doi: 10.1016/j.nano.2024.102753. Online ahead of print.

Authors

Alisa S Postovalova¹, Yulia A Tishchenko², Maria S Istomina³, Timofey E Karpov⁴, Sergei A Shipilovskikh⁵, Daria Akhmetova¹, Anna Rogova⁴, Nina V Gavrilova⁶, Alexander S Timin⁷

Affiliations

¹ Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia; ITMO University, Kronverksky Prospekt 49, bldg. A, St. Petersburg 191002, Russia.
² Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia; St. Petersburg Academic University, St. Petersburg, Russia.
³ Federal State Budgetary Institution "V.A. Almazov National Medical Research Center" of the Ministry of Health of the Russian Federation, St. Petersburg, Russia; St. Petersburg State Electrotechnical University "LETI" named after V.I. Ulyanov (Lenin), St. Petersburg, Russia.
⁴ Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia.
⁵ ITMO University, Kronverksky Prospekt 49, bldg. A, St. Petersburg 191002, Russia.
⁶ Labratory of Intracellular Signaling and Transport, Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, St. Petersburg 197376, Russia; Research Complex "Immunobiotechnology and Gene Therapy", Peter the Great St. Petersburg Polytechnic University, Saint-Petersburg 194021, Russia.
⁷ Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia. Electronic address: timin_as@spbstu.ru.

PMID: 38734039
DOI: 10.1016/j.nano.2024.102753

Abstract

In this study, we have considered four types of nanoparticles (NPs): polylactic acid (PLA), gold (Au), calcium carbonate (CaCO₃), and silica (SiO₂) with similar sizes (TEM: 50-110 nm and DLS: 110-140 nm) to examine their passive accumulation in three different tumors: colon (CT26), melanoma (B16-F10), and breast (4T1) cancers. Our results demonstrate that each tumor model showed a different accumulation of NPs, in the following order: CT26 > B16-F10 > 4T1. The Au and PLA NPs were evidently characterized by a higher delivery efficiency in case of CT26 tumors compared to CaCO₃ and SiO₂ NPs. The Au NPs demonstrated the highest accumulation in B16-F10 cells compared to other NPs. These results were verified using SPECT, ex vivo fluorescence bioimaging, direct radiometry and histological analysis. Thus, this work contributes to new knowledge in passive tumor targeting of NPs and can be used for the development of new strategies for delivery of bioactive compounds.

Keywords: Enhanced Permeability and Retention (EPR); Nanoparticles; Passive targeting; Tumor heterogeneity.