Near-infrared fluorescent probe for the imaging of viscosity in fatty liver mice and valuation of drug efficacy

Jing-Jing Chao; Qin-Ting Liao; Ling Hu; Zhi-Qing Wang; Zhen-Zhen Peng; Guo-Jiang Mao; Fen Xu; Yongfei Li; Chun-Yan Li

doi:10.1016/j.talanta.2024.126227

Near-infrared fluorescent probe for the imaging of viscosity in fatty liver mice and valuation of drug efficacy

Talanta. 2024 May 10:276:126227. doi: 10.1016/j.talanta.2024.126227. Online ahead of print.

Authors

Jing-Jing Chao¹, Qin-Ting Liao¹, Ling Hu¹, Zhi-Qing Wang¹, Zhen-Zhen Peng¹, Guo-Jiang Mao², Fen Xu¹, Yongfei Li³, Chun-Yan Li⁴

Affiliations

¹ Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
² Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, PR China.
³ Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China; College of Chemical Engineering, Xiangtan University, Xiangtan, 411105, PR China. Electronic address: liyongfei@xtu.edu.cn.
⁴ Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China. Electronic address: lichunyan@xtu.edu.cn.

PMID: 38733935
DOI: 10.1016/j.talanta.2024.126227

Abstract

Fatty liver disease affects at least 25 percent of the population worldwide and is a severe metabolic syndrome. Viscosity is closely related to fatty liver disease, so it is urgent to develop an effective tool for monitoring viscosity. Herein, a NIR fluorescent probe called MBC-V is developed for imaging viscosity, consisting of dimethylaniline and malonitrile-benzopyran. MBC-V is non-fluorescent in low viscosity solutions due to intramolecular rotation. In high viscosity solution, the intramolecular rotation of MBC-V is suppressed and the fluorescence is triggered. MBC-V has long emission wavelength at 720 nm and large Stokes shift about 160 nm. Moreover, MBC-V can detect changes in cell viscosity in fatty liver cells, and can image the therapeutic effects of drug in fatty liver cells. By taking advantage of NIR emission, MBC-V can be used as an imaging tool for fatty liver disease and a way to evaluate the therapeutic effect of drug for fatty liver disease.

Keywords: Fatty liver disease; Fluorescent probe; TICT mechanism; Therapeutic effects of drug; Viscosity.