Two-dimensional hydrophilic imprinted resin-graphene oxide composite for selective extraction and rapid determination of gibberellin traces in licorice samples

Mingwei Wang; Yanan Yuan; Yehong Han; Fengxia Qiao; Jinliang Li; Hongyuan Yan

doi:10.1016/j.foodchem.2024.139553

Two-dimensional hydrophilic imprinted resin-graphene oxide composite for selective extraction and rapid determination of gibberellin traces in licorice samples

Food Chem. 2024 May 7:452:139553. doi: 10.1016/j.foodchem.2024.139553. Online ahead of print.

Authors

Mingwei Wang¹, Yanan Yuan¹, Yehong Han¹, Fengxia Qiao², Jinliang Li³, Hongyuan Yan⁴

Affiliations

¹ Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China.
² College of Biochemical and Environmental Engineering, Baoding University, Baoding, 071002, China.
³ Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
⁴ Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China; State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China. Electronic address: yanhy@hbu.edu.cn.

PMID: 38733687
DOI: 10.1016/j.foodchem.2024.139553

Abstract

This study presents novel methodologies and materials for selectively and sensitively determining gibberellin traces in licorice to address food safety concerns. A novel hydrophilic imprinted resin-graphene oxide composite (HMIR-GO) was developed with fast mass transfer, high adsorption capacity, and exceptional aqueous recognition performance for gibberellin. Leveraging the advantages of molecular imprinting, hydrophilic resin synthesis, and rapid mass transfer characteristics of GO, HMIR-GO was employed as an adsorbent, showing resistance to matrix interference. Coupled with HPLC, a rapid and selective method for determining gibberellin was established. Under optimal conditions, the method exhibited a wide linear range (0.02-5.00 μg g^-1, r = 0.9999), low detection limits (3.3 ng g^-1), and satisfactory recoveries (92.0-98.4%), enabling the accurate and rapid detection of gibberellin in licorice. This study introduces a pioneering strategy for the selective extraction and determination of trace gibberellin levels, offering insights for similar applications in functional foods.

Keywords: Aqueous recognition; Functional food; Gibberellin; Hydrophilic imprinted resin-graphene oxide composite; Surface imprinting.