Molecular and Cytological Identification of Wheat- Thinopyrum intermedium Partial Amphiploid Line 92048 with Resistance to Stripe Rust and Fusarium Head Blight

Xiaoqin Luo; Yuanjiang He; Xianli Feng; Min Huang; Kebing Huang; Xin Li; Suizhuang Yang; Yong Ren

doi:10.3390/plants13091198

Molecular and Cytological Identification of Wheat- Thinopyrum intermedium Partial Amphiploid Line 92048 with Resistance to Stripe Rust and Fusarium Head Blight

Plants (Basel). 2024 Apr 25;13(9):1198. doi: 10.3390/plants13091198.

Authors

Xiaoqin Luo¹, Yuanjiang He², Xianli Feng¹, Min Huang¹, Kebing Huang¹, Xin Li¹, Suizhuang Yang¹, Yong Ren²

Affiliations

¹ Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
² Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang Institute of Agricultural Science, Mianyang 621023, China.

Abstract

Thinopyrum intermedium (2n = 6x = 42, E^eE^eE^bE^bStSt or JJJ^sJ^sStSt) contains a large number of genes that are highly adaptable to the environment and immune to a variety of wheat diseases, such as powdery mildew, rust, and yellow dwarf, making it an important gene source for the genetic improvement of common wheat. Currently, an important issue plaguing wheat production and breeding is the spread of pests and illnesses. Breeding disease-resistant wheat varieties using disease-resistant genes is currently the most effective measure to solve this problem. Moreover, alien resistance genes often have a stronger disease-resistant effect than the resistance genes found in common wheat. In this study, the wheat-Th. intermedium partial amphiploid line 92048 was developed through hybridization between Th. intermedium and common wheat. The chromosome structure and composition of 92048 were analyzed using ND-FISH and molecular marker analysis. The results showed that the chromosome composition of 92048 (Octoploid Trititrigia) was 56 = 42W + 6J + 4J^s + 4St. In addition, we found that 92048 was highly resistant to a mixture of stripe rust races (CYR32, CYR33, and CYR34) during the seedling stage and fusarium head blight (FHB) in the field during the adult plant stage, suggesting that the alien or wheat chromosomes in 92048 had disease-resistant gene(s) to stripe rust and FHB. There is a high probability that the gene(s) for resistance to stripe rust and FHB are from the alien chromosomes. Therefore, 92048 shows promise as a bridge material for transferring superior genes from Th. intermedium to common wheat and improving disease resistance in common wheat.

Keywords: Th. intermedium; fusarium head blight (FHB); nondenaturing fluorescence in situ hybridization (ND-FISH); partial amphiploid line; stripe rust.

Grants and funding

This study was financially supported by the Breakthrough in Wheat Breeding Material and Method Innovation and New Variety Breeding (Breeding Research Project, 2021YFYZ0002) and the Major Program of National Agricultural Science and Technology of China (NK20220607).