Treatment of Donor Cells with Oxidative Phosphorylation Inhibitor CPI Enhances Porcine Cloned Embryo Development

Jinping Cao; Yazheng Dong; Zheng Li; Shunbo Wang; Zhenfang Wu; Enqin Zheng; Zicong Li

doi:10.3390/ani14091362

Treatment of Donor Cells with Oxidative Phosphorylation Inhibitor CPI Enhances Porcine Cloned Embryo Development

Animals (Basel). 2024 Apr 30;14(9):1362. doi: 10.3390/ani14091362.

Authors

Jinping Cao^{1

2

3

4

5

6}, Yazheng Dong^{1

2

3

4

5

6}, Zheng Li^{1

2

3

4

5

6}, Shunbo Wang^{1

2

3

4

5

6}, Zhenfang Wu^{1

2

3

4

5

6}, Enqin Zheng^{1

2

3

4

5

6}, Zicong Li^{1

2

3

4

5

6}

Affiliations

¹ National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
² State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural University, Guangzhou 510642, China.
³ National and Local Joint Engineering Research Center for Livestock and Poultry Breeding Industry, South China Agricultural University, Guangzhou 510642, China.
⁴ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
⁵ Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
⁶ Gene Bank of Guangdong Local Livestock and Poultry, South China Agricultural University, Guangzhou 510642, China.

Abstract

Somatic cell nuclear transfer (SCNT) technology holds great promise for livestock industry, life science and human biomedicine. However, the development and application of this technology is limited by the low developmental potential of SCNT embryos. The developmental competence of cloned embryos is influenced by the energy metabolic status of donor cells. The purpose of this study was to investigate the effects of CPI, an oxidative phosphorylation inhibitor, on the energy metabolism pathways of pig fibroblasts and the development of subsequent SCNT embryos. The results showed that treatment of porcine fibroblasts with CPI changed the cellular energy metabolic pathways from oxidative phosphorylation to glycolysis and enhanced the developmental ability of subsequent SCNT embryos. The present study establishes a simple, new way to improve pig cloning efficiency, helping to promote the development and application of pig SCNT technology.

Keywords: CPI; SCNT; glycolysis; oxidative phosphorylation.

Abstract

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