Study on the mechanisms by which pumpkin polysaccharides regulate abnormal glucose and lipid metabolism in diabetic mice under oxidative stress

Zeen Yang; Shengdong Chen; Wenxuan Sun; Yechen Yang; Yuxuan Xu; Yuxuan Tang; Wen Jiang; Jia Li; Yongjun Zhang

doi:10.1016/j.ijbiomac.2024.132249

Study on the mechanisms by which pumpkin polysaccharides regulate abnormal glucose and lipid metabolism in diabetic mice under oxidative stress

Int J Biol Macromol. 2024 May 9;270(Pt 2):132249. doi: 10.1016/j.ijbiomac.2024.132249. Online ahead of print.

Authors

Zeen Yang¹, Shengdong Chen¹, Wenxuan Sun², Yechen Yang¹, Yuxuan Xu², Yuxuan Tang¹, Wen Jiang¹, Jia Li¹, Yongjun Zhang³

Affiliations

¹ College of Life Sciences, Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, Zhejiang Province 310018, China.
² Liang Xin College, China Jiliang University, Hangzhou, Zhejiang Province 310018, China.
³ College of Life Sciences, Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, Zhejiang Province 310018, China. Electronic address: yjzhang@vip.163.com.

PMID: 38729500
DOI: 10.1016/j.ijbiomac.2024.132249

Abstract

Pumpkin polysaccharide (PPe-H) can perform physiological functions through its antioxidative and hypoglycemic effects; however, the mechanisms through which PPe-H regulates abnormal glucose and lipid metabolism caused by oxidative stress injury remain unclear. In the present study, streptozotocin was used to generate an acute diabetic mouse model, and the effects of PPe-H on glucose and lipid metabolism impaired by oxidative stress in diabetic mice were studied. PPe-H significantly reduced blood glucose levels and enhanced the oral glucose tolerance of diabetic mice under stress injury (p < 0.05). The analysis of liver antioxidant enzymes showed that PPe-H significantly enhanced the activities of SOD and CAT (p < 0.05), increased the GSH level, and decreased the level of MDA (p < 0.05). Transcriptomic and metabolomic analyses of the liver tissues of mice revealed characteristic differences in the genetic and metabolic levels of the samples, which showed that PPe-H treatment may play a positive role in regulating the metabolism of methionine, cysteine, glycerol phospholipid, and linoleic acid. These results indicated that PPe-H alleviated the symptoms of hyperglycemia by regulating metabolites related to oxidative stress and glycolipid metabolism in diabetic mice.

Keywords: Metabolomics; Pumpkin polysaccharide; Transcriptomics.