Curcumin and gallic acid have a synergistic protective effect against ovarian surface epithelium and follicle reserve damage caused by autologous intraperitoneal ovary transplantation in rats

Kubra Basol Baki; Tansel Sapmaz; Kubra Sevgin; Sude Topkaraoglu; Esra Erdem; Muhammetnur Tekayev; Eray Metin Guler; Hakan Beyaztas; Kubra Bozali; Selman Aktas; Oktay Irkorucu

doi:10.1016/j.prp.2024.155320

Curcumin and gallic acid have a synergistic protective effect against ovarian surface epithelium and follicle reserve damage caused by autologous intraperitoneal ovary transplantation in rats

Pathol Res Pract. 2024 Apr 23:258:155320. doi: 10.1016/j.prp.2024.155320. Online ahead of print.

Authors

Affiliations

¹ University of Health Sciences, Hamidiye Institute of Health Sciences, Department of Histology and Embryology, Istanbul 34668, Türkiye; Bezmialem Vakif University, Medical Faculty, Department of Histology and Embryology, Istanbul, Türkiye.
² University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul 34668, Türkiye. Electronic address: sapmaz.tansel@gmail.com.
³ University of Health Sciences, International Faculty of Medicine, Department of Histology and Embryology, Istanbul 34668, Türkiye.
⁴ University of Health Sciences, Hamidiye Institute of Health Sciences, Department of Histology and Embryology, Istanbul 34668, Türkiye; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul 34668, Türkiye.
⁵ University of Health Sciences, Vocational School of Health Services, Department of Medical Services and Techniques, Pathology Laboratory Techniques Program, Istanbul 34668, Türkiye.
⁶ University of Health Sciences, Hamidiye Institute of Health Sciences, Department of Histology and Embryology, Istanbul 34668, Türkiye.
⁷ University of Health Sciences, Hamidiye Faculty of Medicine, Haydarpasa Numune Health Application and Research Center, Department of Medical Biochemistry, Istanbul, Türkiye; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Türkiye.
⁸ University of Health Sciences, Hamidiye Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Türkiye.
⁹ University of Health Sciences, Hamidiye Faculty of Medicine, Department of Biostatistics and Medical Informatics, Istanbul, Türkiye.
¹⁰ University of Sharjah, College of Medicine, Department of Clinical Sciences, Sharjah, United Arab Emirates.

PMID: 38728794
DOI: 10.1016/j.prp.2024.155320

Abstract

The objective of this study to examine the effects of curcumin and gallic acid use against oxidative stress damage in the autologous intraperitoneal ovarian transplantation model created in rats on ovarian follicle reserve, ovarian surface epithelium, and oxidant-antioxidant systems. 42 adult female Sprague Dawley rats (n=7) were allocated into 6 groups. Group 1 served as the control. In Group 2, rats underwent ovarian transplantation (TR) to their peritoneal walls. Group 3 received corn oil (CO) (0.5 ml/day) one day before and 14 days after transplantation. Group 4 was administered curcumin (CUR) (100 mg/kg/day), Group 5 received gallic acid (GA) (20 mg/kg/day), and Group 6 was treated with a combination of curcumin and gallic acid via oral gavage after transplantation. Rats were sacrificed on the 14th postoperative day, and blood along with ovaries were collected for analysis. The removed ovaries were analyzed at light microscopic, fluorescence microscopic, and biochemical levels. In Group 2 and Group 3, while serum and tissue Total Oxidant Levels (TOS) and Oxidative Stress Index (OSI) increased, serum Total Antioxidant Levels (TAS) decreased statistically significantly (p˂0.05) compared to the other groups (Groups 1, 4, 5, and 6). The ovarian follicle reserve was preserved and the changes in the ovarian surface epithelium and histopathological findings were reduced in the antioxidant-treated groups (Groups 4, 5, and 6). In addition, immunofluorescence examination revealed that the expression of Cytochrome C and Caspase 3 was stronger and Ki-67 was weaker in Groups 2 and 3, in comparison to the groups that were given antioxidants. It can be said that curcumin and gallic acid have a histological and biochemical protective effect against ischemia-reperfusion injury due to ovarian transplantation, and this effect is stronger when these two antioxidants are applied together compared to individual use.

Keywords: Curcumin; Ovarian follicle reserve; Ovarian transplantation; Oxidative stress; gallic acid.