Genetic dissection of the bacterial Fe-S protein biogenesis machineries

Mathieu Sourice; Charlotte Oriol; Corinne Aubert; Pierre Mandin; Béatrice Py

doi:10.1016/j.bbamcr.2024.119746

Genetic dissection of the bacterial Fe-S protein biogenesis machineries

Biochim Biophys Acta Mol Cell Res. 2024 May 6;1871(5):119746. doi: 10.1016/j.bbamcr.2024.119746. Online ahead of print.

Authors

Mathieu Sourice¹, Charlotte Oriol¹, Corinne Aubert¹, Pierre Mandin¹, Béatrice Py²

Affiliations

¹ Laboratoire de Chimie Bactérienne (UMR7283), Institut de Microbiologie de la Méditerranée, Institut Microbiologie Bioénergies et Biotechnologie, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France.
² Laboratoire de Chimie Bactérienne (UMR7283), Institut de Microbiologie de la Méditerranée, Institut Microbiologie Bioénergies et Biotechnologie, Centre National de la Recherche Scientifique, Aix-Marseille Université, Marseille, France. Electronic address: py@imm.cnrs.fr.

PMID: 38719030
DOI: 10.1016/j.bbamcr.2024.119746

Abstract

Iron‑sulfur (Fe-S) clusters are one of the most ancient and versatile inorganic cofactors present in the three domains of life. Fe-S clusters are essential cofactors for the activity of a large variety of metalloproteins that play crucial physiological roles. Fe-S protein biogenesis is a complex process that starts with the acquisition of the elements (iron and sulfur atoms) and their assembly into an Fe-S cluster that is subsequently inserted into the target proteins. The Fe-S protein biogenesis is ensured by multiproteic systems conserved across all domains of life. Here, we provide an overview on how bacterial genetics approaches have permitted to reveal and dissect the Fe-S protein biogenesis process in vivo.

Keywords: Bacterial genetics; ISC; MIS; NIF; SMS; SUF.

Publication types

Review