Effect of arginine supplementation on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

Shahna Fathima; Walid G Al Hakeem; Revathi Shanmugasundaram; Ramesh K Selvaraj

doi:10.1016/j.psj.2024.103815

Effect of arginine supplementation on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

Poult Sci. 2024 Apr 27;103(7):103815. doi: 10.1016/j.psj.2024.103815. Online ahead of print.

Authors

Shahna Fathima¹, Walid G Al Hakeem¹, Revathi Shanmugasundaram², Ramesh K Selvaraj³

Affiliations

¹ Department of Poultry Science, University of Georgia, Athens, GA, USA.
² Toxicology and Mycotoxin Research Unit, U.S. National Poultry Research Center, United States Department of Agriculture, Athens, GA 30605, USA.
³ Department of Poultry Science, University of Georgia, Athens, GA, USA. Electronic address: selvaraj@uga.edu.

Abstract

The objective of this study was to evaluate the effect of 25% arginine supplementation as a functional amino acid in partially alleviating the detrimental effects of necrotic enteritis (NE) on the growth performance, serum biochemistry, gut integrity, and the relative gene expression of tight junction proteins and inflammatory cytokines in broilers during NE. Three hundred and sixty 1-day-old chicks were randomly allocated to 4 treatments in a 2 × 2 factorial arrangement -basal diet and 125% arginine diet, with or without NE challenge. NE was induced by inoculating 1 × 10⁴Eimeria maxima sporulated oocysts on d 14 and 1 × 10⁸ CFU/bird C. perfringens on d 19, 20, and 21. The NE challenge had a significant effect on the BWG (p < 0.05), FCR (p < 0.05), serum AST (p < 0.05), GLU (p < 0.05), and K⁺ (p < 0.05) levels, and intestinal permeability (p < 0.05) and jejunal lesion score (p < 0.05). A significant challenge × diet interaction effect was observed in the cecal tonsil CD8⁺: CD4⁺ T-cell ratio on d 21 (p < 0.05) and 28 (p < 0.05) and spleen CD8⁺: CD4⁺ T-cell ratio on d 21 (p < 0.05) and 35 (p < 0.05). Arginine supplementation significantly increased the CD8⁺: CD4⁺ T-cell ratio in uninfected birds but decreased the CD8⁺: CD4⁺ T-cell ratio in infected birds. On d 21, a significant interaction effect was observed on the relative expression of the iNOS gene (p < 0.05). Arginine supplementation significantly downregulated the expression of the iNOS gene in infected birds. A significant effect of the challenge (p < 0.05) was observed on the relative gene expression of the ZO-1 gene in the jejunum. NE challenge significantly downregulated the expression of the ZO-1 gene on d 21. In conclusion, arginine supplementation did not alleviate the depression in growth performance and disease severity during the NE challenge. However, arginine downregulated the expression of inflammatory cytokines and enzymes, preventing inflammatory injury to the tissues during NE. Hence, arginine might be supplemented with other alternatives to downregulate inflammatory response during NE in poultry.

Keywords: arginine; broiler; immune response; necrotic enteritis.