Chitin quantitation (as glucosamine) in food raw materials by HPLC-C18-DAD with off-line derivatization

Miloslav Šulc

doi:10.1016/j.mex.2024.102729

Chitin quantitation (as glucosamine) in food raw materials by HPLC-C18-DAD with off-line derivatization

MethodsX. 2024 Apr 22:12:102729. doi: 10.1016/j.mex.2024.102729. eCollection 2024 Jun.

Author

Miloslav Šulc¹

Affiliation

¹ Food Research Institute Prague, Radiova 1285/7, CZ-10200, Prague, Czech Republic.

Abstract

This HPLC method is suitable for chitin quantitation (reported as glucosamine) in food raw materials like insects (mealworm larvae, crickets), shrimps, mushrooms and fungi in a research (non-routine) laboratory using a C18 column with HPLC system <600 bar with UV detection capability (at 265 nm). To remove interferences, the sample is defatted (Soxhlet) and deproteinized (by alkali) prior to acid hydrolysis in 6 M HCl. A five-point linear calibration (5-100 µg/mL) is used. The use of fluorescence detection (λ_ex = 260 nm, λ_em = 350 nm) is also possible with this method [1].•18 min HPLC run time•LOD = 0.05 µg/mL and LOQ = 5 µg/mL.

Keywords: Chitin quantitation in food raw materials by HPLC—C18-DAD with off-line derivatization; Chitosan; Crab; Cricket; Crustaceans; DAD; Diode-array detector; FLD; Fluorescence detector; Fungi; Insects; Liquid chromatography; Lobster; Mealworm; Mushroom; PDA; Reverse phase; Shrimp; Tenebrio molitor.