SARS-CoV-2 cellular and humoral responses in vaccine-naive individuals during the first two waves of COVID-19 infections in the southern region of The Netherlands: a cross-sectional population-based study

D A T Hanssen; K Arts; W H V Nix; N N B Sweelssen; T T J Welbers; C de Theije; L Wieten; D M E Pagen; S Brinkhues; J Penders; N H T M Dukers-Muijrers; C J P A Hoebe; P H M Savelkoul; I H M van Loo

doi:10.1128/spectrum.00126-24

SARS-CoV-2 cellular and humoral responses in vaccine-naive individuals during the first two waves of COVID-19 infections in the southern region of The Netherlands: a cross-sectional population-based study

Microbiol Spectr. 2024 Apr 30:e0012624. doi: 10.1128/spectrum.00126-24. Online ahead of print.

Authors

D A T Hanssen^{1

2}, K Arts¹, W H V Nix¹, N N B Sweelssen¹, T T J Welbers¹, C de Theije³, L Wieten⁴, D M E Pagen^{2

5

6}, S Brinkhues⁷, J Penders^{1

2

8}, N H T M Dukers-Muijrers^{2

5

9}, C J P A Hoebe^{1

2

5

6}, P H M Savelkoul^{1

2}, I H M van Loo^{1

2}

Affiliations

¹ Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Maastricht University Medical Center, Maastricht, The Netherlands.
² Care and Public Health Research Institute (CAPHRI), Maastricht University, Maastricht, The Netherlands.
³ BioBank Maastricht UMC+, Maastricht University Medical Center, Maastricht, The Netherlands.
⁴ Department of Transplantation Immunology, Maastricht University Medical Center, Maastricht, The Netherlands.
⁵ Department of Sexual Health, Infectious Diseases and Environmental Health, Living Lab Public Health, Public Health Service (GGD) South Limburg, Heerlen, The Netherlands.
⁶ Department of Social Medicine, Maastricht University, Maastricht, The Netherlands.
⁷ Department of Knowledge and Innovation, Public Health Service (GGD) South Limburg, Heerlen, The Netherlands.
⁸ Department of Medical Microbiology, School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University Medical Center, Maastricht, The Netherlands.
⁹ Department of Health Promotion, Maastricht University, Maastricht, The Netherlands.

PMID: 38686954
DOI: 10.1128/spectrum.00126-24

Abstract

With the emergence of highly transmissible variants of concern, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) still poses a global threat of coronavirus disease 2019 (COVID-19) resurgence. Cellular responses to novel variants are more robustly maintained than humoral responses, and therefore, cellular responses are of interest in assessing immune protection against severe disease in the population. We aimed to assess cellular responses to SARS-CoV-2 at the population level. IFN_γ (interferon _γ) responses to wild-type SARS-CoV-2 were analyzed using an ELISpot assay in vaccine-naive individuals with different humoral responses: Ig (IgM and/or IgG) seronegative (n = 90) and seropositive (n = 181) with low (<300 U/mL) or high (≥300 U/mL) humoral responses to the spike receptor binding domain (anti-S-RBD). Among the seropositive participants, 71.3% (129/181) were IFN_γ ELISpot positive, compared to 15.6% (14/90) among the seronegative participants. Common COVID-19 symptoms such as fever and ageusia were associated with IFN_γ ELISpot positivity in seropositive participants, whereas no participant characteristics were associated with IFN_γ ELISpot positivity in seronegative participants. Fever and/or dyspnea and anti-S-RBD levels were associated with higher IFN_γ responses. Symptoms of more severe disease and higher anti-S-RBD responses were associated with higher IFN_γ responses. A significant proportion (15.6%) of seronegative participants had a positive IFN_γ ELISpot. Assessment of cellular responses may improve estimates of the immune response to SARS-CoV-2 in the general population.

Importance: Data on adaptive cellular immunity are of interest to define immune protection against severe acute respiratory syndrome coronavirus 2 in a population, which is important for decision-making on booster-vaccination strategies. This study provides data on associations between participant characteristics and cellular immune responses in vaccine-naive individuals with different humoral responses.

Keywords: ELISpot; IFNγ response; SARS-CoV-2; antibody response; cellular immunity; peripheral blood mononuclear cell.