An AIE-based fluorescent probe to detect peroxynitrite levels in human serum and its cellular imaging

Chem Commun (Camb). 2024 May 16;60(41):5443-5446. doi: 10.1039/d4cc01231c.

Abstract

An AIE-based fluorescent probe was designed to evaluate peroxynitrite levels in complex biological samples. The newly synthesized hydrazone-conjugated probe fluoresces strongly in the presence of peroxynitrite. Clinically, the peroxynitrite levels can be measured in human serum and cellular mitochondria with an LOD of 6.5 nM by fluorescence imaging in vitro.

MeSH terms

  • Fluorescent Dyes* / chemical synthesis
  • Fluorescent Dyes* / chemistry
  • HeLa Cells
  • Humans
  • Hydrazones / chemical synthesis
  • Hydrazones / chemistry
  • Limit of Detection
  • Mitochondria / chemistry
  • Mitochondria / metabolism
  • Molecular Structure
  • Optical Imaging*
  • Peroxynitrous Acid* / analysis
  • Peroxynitrous Acid* / blood