Previous studies have shown that growth arrest, dedifferentiation, and loss of original function occur in cells after multiple generations of culture, which are attributed to the lack of stress stimulation. To investigate the effects of multi-modal biomimetic stress (MMBS) on the biological function of human bladder smooth muscle cells (HBSMCs), a MMBS culture system was established to simulate the stress environment suffered by the bladder, and HBSMCs were loaded with different biomimetic stress for 24 h. Then, cell growth, proliferation and functional differentiation were detected. The results showed that MMBS promoted the growth and proliferation of HBSMCs, and 80 cm H 2O pressure with 4% stretch stress were the most effective in promoting the growth and proliferation of HBSMCs and the expression level of α-smooth muscle actin and smooth muscle protein 22-α. These results suggest that the MMBS culture system will be beneficial in regulating the growth and functional differentiation of HBSMCs in the construction of tissue engineered bladder.
以往研究表明，经过多代培养后的细胞会出现生长停滞、去分化和丧失原有功能等问题，其原因是缺乏应力刺激。为探讨多模化仿生应力（MMBS）对人膀胱平滑肌细胞（HBSMCs）生物学功能的影响，本实验建立了MMBS培养系统来模拟膀胱所受的应力环境，并对HBSMCs加载不同强度的仿生应力持续24 h，然后检测细胞生长、增殖及功能分化。结果显示，MMBS可以促进HBSMCs的生长和增殖，以80 cm H 2O压力附加4%牵张应力，对促进HBSMCs的生长、增殖以及α—平滑肌肌动蛋白和平滑肌蛋白22—α的表达最有效。综上，在构建组织工程膀胱时，MMBS培养系统将有助于调节HBSMCs的生长、增殖以及功能分化成熟。.
Keywords: Bladder smooth muscle cells; Cell differentiation; Cell proliferation; Stress; Tissue engineering.