Ceftiofur in swine manure contributes to reducing pathogens and antibiotic resistance genes during composting

Wenjin Ma; Boyu An; Xiangyue Xu; Meixia Huo; Kun Mi; Xiaoyuan Tian; Ziyan Kou; Aoran Tang; Guyue Cheng; Lingli Huang

doi:10.1016/j.envres.2024.119033

Ceftiofur in swine manure contributes to reducing pathogens and antibiotic resistance genes during composting

Environ Res. 2024 Apr 27;252(Pt 3):119033. doi: 10.1016/j.envres.2024.119033. Online ahead of print.

Authors

Wenjin Ma¹, Boyu An¹, Xiangyue Xu¹, Meixia Huo¹, Kun Mi², Xiaoyuan Tian², Ziyan Kou¹, Aoran Tang¹, Guyue Cheng³, Lingli Huang⁴

Affiliations

¹ National Laboratory for Veterinary Drug Safety Evaluation, Huazhong Agriculture University, Wuhan 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China.
² National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agriculture University, Wuhan 430070, China; MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China.
³ National Laboratory for Veterinary Drug Safety Evaluation, Huazhong Agriculture University, Wuhan 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei 430070, China; National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agriculture University, Wuhan 430070, China; College of Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China. Electronic address: chengguyue@mail.hzau.edu.cn.
⁴ National Laboratory for Veterinary Drug Safety Evaluation, Huazhong Agriculture University, Wuhan 430070, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei 430070, China; National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agriculture University, Wuhan 430070, China; MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei 430070, China; College of Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China. Electronic address: huanglingli@mail.hzau.edu.cn.

PMID: 38685300
DOI: 10.1016/j.envres.2024.119033

Abstract

Aerobic composting is a common way for the disposal of feces produced in animal husbandry, and can reduce the release of antibiotic resistance genes (ARGs) from feces into the environment. In this study, we collected samples from two distinct treatments of swine manure compost with and without ceftiofur (CEF), and identified the ARGs, mobile genetic elements (MGEs), and bacterial community by metagenomic sequencing. The impacts of CEF on the bacterial community composition and fate of ARGs and MGEs were investigated. With increasing composting temperature and pH, the concentration of CEF in the manure decreased rapidly, with a degradation half-life of 1.12 d and a 100% removal rate after 10 d of aerobic composting. Metagenomics demonstrated that CEF in the manure might inhibit the growth of Firmicutes and Proteobacteria, thereby reducing some ARGs and MGEs hosted by these two bacteria, which was further confirmed by the variations of ARGs and MGEs. A further redundancy analysis suggested that pH and temperature are key environmental factors affecting ARG removal during composting, and intI1 and bacterial communities also have significant influence on ARG abundance. These results are of great significance for promoting the removal of some ARGs from animal manure by controlling some key environmental factors and the type of antibiotics used in animals.

Keywords: Antibiotic resistance genes; Cephalosporins; Potential host bacteria; Removal efficiency.