Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells

BMC Cardiovasc Disord. 2024 Apr 29;24(1):231. doi: 10.1186/s12872-024-03858-3.

Abstract

Background: Oxidized low-density lipoprotein (ox-LDL) can initiate and affect almost all atherosclerotic events including endothelial dysfunction. In this text, the role and underlying molecular basis of procyanidin B2 (PCB2) with potential anti-oxidant and anti-inflammatory activities in ox-LDL-induced HUVEC injury were examined.

Methods: HUVECs were treated with ox-LDL in the presence or absence of PCB2. Cell viability and apoptotic rate were examined by CCK-8 assay and flow cytometry, respectively. The mRNA and protein levels of genes were tested by RT-qPCR and western blot assays, respectively. Potential downstream targets and pathways of apple procyanidin oligomers were examined by bioinformatics analysis for the GSE9647 dataset. The effect of PCB2 on THP-1 cell migration was examined by recruitment assay. The effect of PCB2 on oxidative stress was assessed by reactive oxygen species (ROS) level, malondialdehyde (MDA) content, and mitochondrial membrane potential (MMP).

Results: ox-LDL reduced cell viability, induced cell apoptosis, and facilitated the expression of oxidized low-density lipoprotein receptor 1 (LOX-1), C-C motif chemokine ligand 2 (MCP-1), vascular cell adhesion protein 1 (VCAM-1) in HUVECs. PCB2 alleviated ox-LDL-induced cell injury in HUVECs. Apple procyanidin oligomers triggered the differential expression of 592 genes in HUVECs (|log2fold-change| > 0.58 and adjusted p-value < 0.05). These dysregulated genes might be implicated in apoptosis, endothelial cell proliferation, inflammation, and monocyte chemotaxis. PCB2 inhibited C-X-C motif chemokine ligand 1/8 (CXCL1/8) expression and THP-1 cell recruitment in ox-LDL-stimulated HUVECs. PCB2 inhibited ox-LDL-induced oxidative stress and nuclear factor kappa-B (NF-κB) activation in HUVECs.

Conclusion: PCB2 weakened ox-LDL-induced cell injury, inflammation, monocyte recruitment, and oxidative stress by inhibiting the NF-κB pathway in HUVECs.

Keywords: Endothelial cells; Inflammation; Monocyte; Oxidative stress; Procyanidin B2; ox-LDL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents* / pharmacology
  • Antioxidants / pharmacology
  • Apoptosis* / drug effects
  • Biflavonoids* / pharmacology
  • Catechin* / pharmacology
  • Chemotaxis, Leukocyte / drug effects
  • Human Umbilical Vein Endothelial Cells* / drug effects
  • Human Umbilical Vein Endothelial Cells* / metabolism
  • Human Umbilical Vein Endothelial Cells* / pathology
  • Humans
  • Lipoproteins, LDL* / toxicity
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Monocytes / pathology
  • NF-kappa B* / metabolism
  • Oxidative Stress* / drug effects
  • Proanthocyanidins* / pharmacology
  • Reactive Oxygen Species / metabolism
  • Scavenger Receptors, Class E / genetics
  • Scavenger Receptors, Class E / metabolism
  • Signal Transduction* / drug effects
  • THP-1 Cells

Substances

  • Lipoproteins, LDL
  • Catechin
  • Proanthocyanidins
  • procyanidin B2
  • Biflavonoids
  • oxidized low density lipoprotein
  • NF-kappa B
  • Anti-Inflammatory Agents
  • Antioxidants
  • OLR1 protein, human
  • Reactive Oxygen Species
  • Scavenger Receptors, Class E