Genome-wide identification of toll-like receptors in Octopus sinensis and expression analysis in response to different PAMPs stimulation

Zebin Chen; Yuquan Zhou; Xinxin Chen; Yinzhen Sheng; Jiaqian Liao; Yicong Huang; Xiao Zhong; Jianming Zhang; Youfang Zhu; Ziping Zhang; Yilei Wang

doi:10.1016/j.fsi.2024.109591

Genome-wide identification of toll-like receptors in Octopus sinensis and expression analysis in response to different PAMPs stimulation

Fish Shellfish Immunol. 2024 Apr 26:149:109591. doi: 10.1016/j.fsi.2024.109591. Online ahead of print.

Authors

Affiliations

¹ Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen, 361021, China.
² Putian Municipal Institute of Fishery Science, Putian, 351100, China.
³ College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: zhangziping@hotmail.com.
⁴ Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, China; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen, 361021, China. Electronic address: ylwang@jmu.edu.cn.

PMID: 38679344
DOI: 10.1016/j.fsi.2024.109591

Abstract

Toll-like receptors (TLRs) are one of the extensively studied pattern recognition receptors (PRRs) and play crucial roles in the immune responses of vertebrates and invertebrates. In this study, 14 TLR genes were identified from the genome-wide data of Octopus sinensis. Protein structural domain analysis showed that most TLR proteins had three main structural domains: extracellular leucine-rich repeats (LRR), transmembrane structural domains, and intracellular Toll/IL-1 receptor domain (TIR). The results of subcellular localization prediction showed that the TLRs of O. sinensis were mainly located on the plasma membrane. The results of quantitative real-time PCR (qPCR) showed that the detected TLR genes were differentially expressed in the hemolymph, white bodies, hepatopancreas, gills, gill heart, intestine, kidney, and salivary gland of O. sinensis. Furthermore, the present study investigated the expression changes of O. sinensis TLR genes in hemolymph, white bodies, gills, and hepatopancreas in different phases (6 h, 12 h, 24 h, 48 h) after stimulation with PGN, poly(I: C) and Vibrio parahaemolyticus. The expression of most of the TLR genes was upregulated at different time points after infection with pathogens or stimulation with PAMPs, a few genes were unchanged or even down-regulated, and many of the TLR genes were much higher after V. parahaemolyticus infection than after PGN and poly(I:C) stimulation. The results of this study contribute to a better understanding of the molecular immune mechanisms of O. sinensis TLRs genes in resistance to pathogen stimulation.

Keywords: Immune response; Octopus sinensis; PGN; TLRs; Vibrio parahaemolyticus; poly(I:C).