Enzymatic fluorescent supramolecular hydrogel with aggregation-induced emission characteristics for sensing alkaline phosphatase

Yun Deng; Cheng Fu; Aifei Xu; Rongxiang He; Wangting Lu; Min Liu

doi:10.1016/j.saa.2024.124334

Enzymatic fluorescent supramolecular hydrogel with aggregation-induced emission characteristics for sensing alkaline phosphatase

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Aug 5:316:124334. doi: 10.1016/j.saa.2024.124334. Epub 2024 Apr 23.

Authors

Yun Deng¹, Cheng Fu², Aifei Xu³, Rongxiang He², Wangting Lu², Min Liu⁴

Affiliations

¹ Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China. Electronic address: ustcdy@jhun.edu.cn.
² Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
³ School of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, China.
⁴ Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China. Electronic address: mliu2018@jhun.edu.cn.

PMID: 38678837
DOI: 10.1016/j.saa.2024.124334

Abstract

Alkaline phosphatase is an important biomarker for medical diagnosis. An enzymatic fluorescence supramolecular hydrogel with AIE properties was developed and used for sensing alkaline phosphatase in vitro and in living cells. In the presence of ALP, K(TPE)EFYp was partially converted to the hydrogelator K(TPE)EFY and self-assembled into nanofibers to form Hydrogel. With the sol-gel transition and the AIE effect, the fluorescence emission was turned on. The linear concentration range of ALP activity in vitro quantified by this method was determined as 0-3 U/L with aLODat 0.02 U/L. In addition, cell imaging and serum experiment showed that K(TPE)EFYp could also be used to detect ALP activity in living cells and biological samples.

Keywords: Aggregation-induced emission; Alkaline phosphatase; Fluorescent supramolecular hydrogel.

MeSH terms

Alkaline Phosphatase* / analysis
Alkaline Phosphatase* / metabolism
Fluorescent Dyes / chemistry
Humans
Hydrogels* / chemistry
Spectrometry, Fluorescence*

Substances

Alkaline Phosphatase
Hydrogels
Fluorescent Dyes