Development of a UPLC-MS/MS-based method for simultaneous determination of advanced glycation end products and heterocyclic amines in stewed meat products

Tan Wang; Maofeng Dong; Qinyi Shen; Guangyue Wen; Mingfu Wang; Yueliang Zhao

doi:10.1016/j.foodchem.2024.139470

Development of a UPLC-MS/MS-based method for simultaneous determination of advanced glycation end products and heterocyclic amines in stewed meat products

Food Chem. 2024 Apr 23:451:139470. doi: 10.1016/j.foodchem.2024.139470. Online ahead of print.

Authors

Tan Wang¹, Maofeng Dong², Qinyi Shen², Guangyue Wen², Mingfu Wang³, Yueliang Zhao⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs/ Pesticide Safety Evaluation Research Center, Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
² Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs/ Pesticide Safety Evaluation Research Center, Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
³ Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen, China. Electronic address: mfwang@szu.edu.cn.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: ylzhao1@sjtu.edu.cn.

PMID: 38678663
DOI: 10.1016/j.foodchem.2024.139470

Abstract

A novel analytical strategy was proposed to simultaneously quantify two advanced glycation end products (AGEs) including N^ε-(Carboxymethyl)lysine (CML), N^ε-(Carboxyethyl)lysine (CEL) and eight heterocyclic amines (HAs) including IQ, MeIQ, MeIQx, 4,8-DiMeIQx, 7,8-DiMeIQx, PhIP, Harman, and Norharman. The procedure was based on a two-step extraction, solid phase extraction (SPE) purification followed by ultra performance liquid chromatography tandem mass spectrometry. The established method showed a good linearity (R² ≥ 0.9950), rapid processing time (8 min per sample), satisfactory recoveries (matrix spiked recoveries range from 72.2% to 119.6%) and precision (intra-day and inter-day RSDs were <19.3%). The limit of quantification (LOQ) and limit of detection (LOD) resulted to be between 0.05-15 ng/g and 0.2-50 ng/g, respectively. The validated technique was further applied to determine HAs and AGEs in eight stewed meat product samples consumed in Shanghai, with the amount of HAs and AGEs ranging from 2.851 to 18.289 ng/g and 118.158-543.493 ng/g, respectively.

Keywords: AGEs; HAs; Stewed meat products; UPLC-MS/MS.