Structural characterization of the polysaccharide from the black crystal region of Inonotus obliquus and its effect on AsPC-1 and SW1990 pancreatic cancer cell apoptosis

Miao Ding; Yu Yang; Ziyang Zhang; Hongxiang Liu; Yingdi Dai; Zixuan Wang; Sijia Ma; Yang Liu; Qi Wang

doi:10.1016/j.ijbiomac.2024.131891

Structural characterization of the polysaccharide from the black crystal region of Inonotus obliquus and its effect on AsPC-1 and SW1990 pancreatic cancer cell apoptosis

Int J Biol Macromol. 2024 Apr 25;268(Pt 2):131891. doi: 10.1016/j.ijbiomac.2024.131891. Online ahead of print.

Authors

Miao Ding¹, Yu Yang², Ziyang Zhang³, Hongxiang Liu¹, Yingdi Dai¹, Zixuan Wang¹, Sijia Ma¹, Yang Liu⁴, Qi Wang⁵

Affiliations

¹ Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China; College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
² Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China; College of Life Science, Jilin Agricultural University, Changchun 130118, China.
³ College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
⁴ Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China; College of Plant Protection, Jilin Agricultural University, Changchun 130118, China. Electronic address: y_liu10@jlau.edu.cn.
⁵ Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China; College of Plant Protection, Jilin Agricultural University, Changchun 130118, China. Electronic address: qiwang@jlau.edu.cn.

PMID: 38677687
DOI: 10.1016/j.ijbiomac.2024.131891

Abstract

In this study, one water soluble polysaccharide (IOP1-1) with a weight average molecular weight of 6886 Da was obtained from the black crystal region of Inonotus obliquus by hot water extraction, DEAE-52 cellulose extraction and Sephadex-100 column chromatography purification. Structural analysis indicated that IOP1-1 was a glucan with a main chain composed of α-Glcp-(1 → 4)-α-Glcp-(1 → 4)-β-Glcp-(1 → 4)-β-Glcp-(1 → 4)-α-Glcp-(1 → 6)-β-Glcp-(1 → 4)-α-Glcp-(1 → 3)-β-Glcp-(1→. The CCK-8 assay results showed that IOP1-1 inhibited AsPC-1 and SW1990 pancreatic cancer cell proliferation in a concentration-dependent manner. Flow cytometric analysis revealed that IOP1-1 induced cell cycle arrest in AsPC-1 and SW1990 cells. Hoechst 33342 staining and Annexin V-FITC/PI double staining analysis showed that IOP1-1 could induce apoptosis in AsPC-1 and SW1990 cells. Furthermore, western blot analysis confirmed that IOP1-1 could induce apoptosis in AsPC-1 and SW1990 pancreatic cancer cells through three pathways: the mitochondrial pathway, the death receptor pathway, and endoplasmic reticulum stress. According to these research data, IOP1-1 may be utilized as an adjuvant treatment to anticancer medications, opening up new application prospects and opportunities.

Keywords: Apoptosis; Inonotus obliquus polysaccharide; Pancreatic cancer cells; Structural characterization.