Toxicity, biochemical and molecular docking studies of Acacia nilotica L., essential oils against insect pests

Perumal Vivekanandhan; Sulaiman Ali Alharbi; Mohammad Javed Ansari

doi:10.1016/j.toxicon.2024.107737

Toxicity, biochemical and molecular docking studies of Acacia nilotica L., essential oils against insect pests

Toxicon. 2024 Apr 26:243:107737. doi: 10.1016/j.toxicon.2024.107737. Online ahead of print.

Authors

Perumal Vivekanandhan¹, Sulaiman Ali Alharbi², Mohammad Javed Ansari³

Affiliations

¹ Department of General Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, 600077, India. Electronic address: vivekanandhanp.sdc@saveetha.com.
² Department of Botany & Microbiology College of Science King Saud University, P.O Box 2455, Riyadh, 11451, Saudi Arabia.
³ Department of Botany, Hindu College Moradabad (Mahatma Jyotiba Phule Rohilkhand University Bareilly), India.

PMID: 38677379
DOI: 10.1016/j.toxicon.2024.107737

Abstract

Botanical essential oils are natural insecticides derived from plants, offering eco-friendly alternatives to synthetic chemicals for pest control. In this study, the essential oils were extracted from Acacia nilotica seed cotyledons, and their toxicity was tested against insect pests. Furthermore, the chemical components of the essential oils were identified through gas chromatography-mass spectrometry (GC-MS) analysis. The essential oil extracted from A. nilotica seeds exhibited the highest mortality rates of 60% and 98% in Culex quinquefasciatus, and 60% and 96.66% mortality in Plutella xylostella at 24 and 48 h after treatment, respectively. The essential oils resulted in a lower LC₅₀ of 159.263 ppm/mL, and LC₉₀ of 320.930 ppm/mL within 24 h. In 48 h, the LC₅₀ was 52.070 ppm/mL and the LC₉₀ was 195.123 ppm/mL for C. quinquefasciatus. In the essential oil treatment of P. xylostella, the lower LC₅₀ was 165.900 ppm/mL, and the LC₉₀ was 343.840 ppm/mL 24 h after the treatment. At 48 h post-treatment, the LC₅₀ decreased to 62.965 ppm/mL, and the LC₉₀ decreased to 236.795 ppm/mL in P. xylostella. The study investigated the impact of essential oils on insect enzymes 24 h after treatment. The study revealed significant changes in the levels of insect enzymes, including a decrease in acetylcholinesterase enzymes and an increase in glutathione S-transferase compared to the control group. Essential oils had minimal effects, resulting in mortality rates of 30.66% and 46% at 24 and 48 h after treatment on Artemia salina. After 48 h, minimal toxic effects of essential oils were observed on E. eugeniae, with a mortality rate of 11.33%. The GC-MS analysis of A. nilotica seed-derived essential oils revealed ten major chemical constituents, including 6-hydroxymellein, phthalic acid, trichloroacetic acid, hexadecane, acetamide, heptacosane, eicosane, pentadecane, 1,3,4-eugenol, and chrodrimanin B. Among these constituents, Heptacosane is the major chemical component, and this molecule has a high potential for involvement in insecticidal activity.

Keywords: Culex quinquefasciatus; Essential oil; Larvicidal activity; Non-targeted organisms; Plutella xylostella.