Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Jinyue Sun; Qian-Jun Shen; Jia-Neng Pan; Xiaodong Zheng; Ting Yu; Wen-Wen Zhou

doi:10.1016/j.ultsonch.2024.106884

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

Ultrason Sonochem. 2024 Apr 20:106:106884. doi: 10.1016/j.ultsonch.2024.106884. Online ahead of print.

Authors

Jinyue Sun¹, Qian-Jun Shen¹, Jia-Neng Pan¹, Xiaodong Zheng¹, Ting Yu², Wen-Wen Zhou³

Affiliations

¹ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
² College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: yuting@zju.edu.cn.
³ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: vivianzhou11@zju.edu.cn.

Abstract

The purpose of this study was to investigate ferroptosis in Escherichia coli O157:H7 caused by ferrous sulfate (FeSO₄) and to examine the synergistic effectiveness of FeSO₄ combined with ultrasound-emulsified cinnamaldehyde nanoemulsion (CALNO) on inactivation of E. coli O157:H7 in vitro and in vivo. The results showed that FeSO₄ could cause ferroptosis in E. coli O157:H7 via generating reactive oxygen species (ROS) and exacerbating lipid peroxidation. In addition, the results indicated that FeSO₄ combined with CALNO had synergistic bactericidal effect against E. coli O157:H7 and the combined treatment could lead considerable nucleic acids and protein to release by damaging the cell membrane of E. coli O157:H7. Besides, FeSO₄ combined with CALNO had a strong antibiofilm ability to inhibit E. coli O157:H7 biofilm formation by reducing the expression of genes related on biofilm formation. Finally, FeSO₄ combined with CALNO exhibited the significant antibacterial activity against E. coli O157:H7 in hami melon and cherry tomato.

Keywords: Antibiofilm; Cinnamaldehyde nanoemulsion; E. coli O157:H7; Ferroptosis; Ferrous sulfate.