Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex

Pedro Trevizan-Baú; Mitchell Ty Ringuet; Martin J Stebbing; Robin M McAllen; John B Furness; Scott N Mueller

doi:10.1016/j.xpro.2024.103036

Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex

STAR Protoc. 2024 Apr 26;5(2):103036. doi: 10.1016/j.xpro.2024.103036. Online ahead of print.

Authors

Pedro Trevizan-Baú¹, Mitchell Ty Ringuet², Martin J Stebbing³, Robin M McAllen³, John B Furness⁴, Scott N Mueller⁵

Affiliations

¹ The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia; Department of Physiological Sciences, University of Florida, Gainesville, FL, USA. Electronic address: trevizanbaup@ufl.edu.
² The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia.
³ The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia.
⁴ The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Anatomy and Physiology, University of Melbourne, Parkville, VIC, Australia.
⁵ Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia. Electronic address: smue@unimelb.edu.au.

Abstract

Neurons that originate from pre-vertebral sympathetic ganglia, the splanchnic-celiac-superior mesenteric ganglion complex (SCSMG) in mouse, have important roles in control of organs of the upper abdomen. Here, we present a protocol for the isolation of the mouse sympathetic SCSMG. We describe steps for surgical incision, ganglia isolation, ganglia fine dissection, and whole-mount SCSMG after clearing-enhanced 3D (Ce3D) clearing method and immunohistochemistry. Given the importance of mice in studies of that control, this protocol aims to assist biomedical researchers in the dissection of the mouse SCSMG.

Keywords: Immunology; Neuroscience; Systems biology.