Transcriptional Regulation and Gene Mapping of Internode Elongation and Late Budding in the Chinese Cabbage Mutant lcc

Yunqin Zhang; Shuxin Xuan; Jiaojiao Zhao; Hui Li; Yin Lu; Rui Li; Yanhua Wang; Shuxing Shen; Xiaoxue Sun; Daling Feng

doi:10.3390/plants13081083

Transcriptional Regulation and Gene Mapping of Internode Elongation and Late Budding in the Chinese Cabbage Mutant lcc

Plants (Basel). 2024 Apr 12;13(8):1083. doi: 10.3390/plants13081083.

Authors

Yunqin Zhang¹, Shuxin Xuan², Jiaojiao Zhao², Hui Li², Yin Lu², Rui Li¹, Yanhua Wang², Shuxing Shen², Xiaoxue Sun³, Daling Feng¹

Affiliations

¹ State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, College of Life Science, Hebei Agricultural University, Baoding 071000, China.
² Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, China.
³ State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, China.

Abstract

Two important traits of Chinese cabbage, internode length and budding time, destroy the maintenance of rosette leaves in the vegetative growth stage and affect flowering in the reproductive growth stage. Internodes have received much attention and research in rice due to their effect on lodging resistance, but they are rarely studied in Chinese cabbage. In Chinese cabbage, internode elongation affects not only the maintenance of rosette leaves but also bolting and yield. Budding is also an important characteristic of Chinese cabbage entering reproductive growth. Although many studies have reported on flowering and bolting, studies on bud emergence and the timing of budding are scarce. In this study, the mutant lcc induced by EMS (Ethyl Methane Sulfonate) was used to study internode elongation in the seedling stage and late budding in the budding stage. By comparing the gene expression patterns of mutant lcc and wild-type A03, 2280 differentially expressed genes were identified in the seedling stage, 714 differentially expressed genes were identified in the early budding stage, and 1052 differentially expressed genes were identified in the budding stage. Here, the transcript expression patterns of genes in the plant hormone signaling and clock rhythm pathways were investigated in relation to the regulation of internode elongation and budding in Chinese cabbage. In addition, an F₂ population was constructed with the mutants lcc and R500. A high-density genetic map with 1602 marker loci was created, and QTLs for internode length and budding time were identified. Specifically, five QTLs for internode length and five QTLs for budding time were obtained. According to transcriptome data analysis, the internode length candidate gene BraA02g005840.3C (PIN8) and budding time candidate genes BraA02g003870.3C (HY5-1) and BraA02g005190.3C (CHS-1) were identified. These findings provide insight into the regulation of internode length and budding time in Chinese cabbage.

Keywords: BSA; Chinese cabbage; QTL; budding time; internodes.

Abstract

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