Development of metabolomic biomarkers to discriminate the geographical origin of Korean and Russian snow crabs using CE-TOF/MS

Jiyoung Shin; Junho Yang; Hyunsuk Kim; Yikang Sim; Eunji Cha; Ji-Young Yang

doi:10.1016/j.foodchem.2024.139286

Development of metabolomic biomarkers to discriminate the geographical origin of Korean and Russian snow crabs using CE-TOF/MS

Food Chem. 2024 Apr 10:451:139286. doi: 10.1016/j.foodchem.2024.139286. Online ahead of print.

Authors

Jiyoung Shin¹, Junho Yang¹, Hyunsuk Kim¹, Yikang Sim¹, Eunji Cha¹, Ji-Young Yang²

Affiliations

¹ Department of Food Science & Technology, Pukyong National University, Busan 48513, Republic of Korea.
² Department of Food Science & Technology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: jyyang@pknu.ac.kr.

PMID: 38670021
DOI: 10.1016/j.foodchem.2024.139286

Abstract

The quantity of snow crabs (Chionoecetes opilio) harvested in Korea is subject to seasonal restrictions; therefore, snow crabs are imported from Russia. Metabolites in snow crabs from two geographic origins were compared. The metabolites were subjected to metabolomic analysis to prevent fraudulent sales of marine products from a particular country. Capillary electrophoresis time-of-flight mass spectrometry was used. Seventy-seven target metabolites were identified using a mass spectral library. Through orthogonal partial least squares discriminant analysis, the top 25 biomarker candidates were evaluated based on p-values and fold changes. A total of 246 peaks (187 and 59 in the cation and anion modes, respectively) were identified. Among the biomarker candidates, 2-oxovaleric acid, asymmetric dimethylarginine, hypotaurine, and allo-threonine were selected as final biomarkers to unequivocally determine the geographic origin. Overall, metabolic analyses allowed us to differentiate snow crabs from different geographic origins. This method could also be extended of other marine products.

Keywords: 2-oxovaleric acid; Biomarker development; Capillary electrophoresis time-of-flight mass spectrometry; Chionoecetes opilio; Multivariate analysis.