Target-induced DNA nanomachine operation for the detection of proteins

Jiao Zheng; Songbai Tian; Qizhen Lai; Xinghu Ji; Fuxiang Zhou; Zhike He

doi:10.1016/j.talanta.2024.126143

Target-induced DNA nanomachine operation for the detection of proteins

Talanta. 2024 Apr 23:275:126143. doi: 10.1016/j.talanta.2024.126143. Online ahead of print.

Authors

Jiao Zheng¹, Songbai Tian², Qizhen Lai³, Xinghu Ji³, Fuxiang Zhou⁴, Zhike He⁵

Affiliations

¹ Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Province Cancer Clinical Study Center, 430071, Wuhan, China; College of Chemistry and Molecular Sciences, Wuhan University, 430072, Wuhan, China.
² College of Chemistry and Molecular Sciences, Wuhan University, 430072, Wuhan, China; School of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China.
³ College of Chemistry and Molecular Sciences, Wuhan University, 430072, Wuhan, China.
⁴ Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Province Cancer Clinical Study Center, 430071, Wuhan, China.
⁵ Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Province Cancer Clinical Study Center, 430071, Wuhan, China; College of Chemistry and Molecular Sciences, Wuhan University, 430072, Wuhan, China. Electronic address: zhkhe@whu.edu.cn.

PMID: 38669960
DOI: 10.1016/j.talanta.2024.126143

Abstract

Accurate and sensitive detection of disease-associated proteins in early stage of patients plays an important role in timely treatment and successfully extending patients' lives. To meet this demand, we herein rationally designed a flexible target-induced DNA nanomachine operation (TIDNMO) sensor for the detection of proteins. The TIDNMO system was composed of DNA nanoswitch and DNA walker. Triplex DNA nanoswitch was triggered by specific target, followed by the release of the walking strand, which initiated the DNA walker amplification as signal output. In addition, the Exo III could drive walking strand autonomously move on gold nanoparticle surface to realize 2 orders of magnitude signal amplification. What's more, this sensor could transform its suitable functional recognition element of DNA nanoswitch to recognize other specific molecule and realize different targets sensing based on identical walking tracks. Considering the facile reporter elements and efficient amplification performance, the present DNA nanomachine as a sensor could achieve a detection limit of 68 pM for anti-Dig antibody, 0.95 pM for mucin-1 respectively, along with a superb specificity. Furthermore, the method reported here opened a new chapter in disease-related protein sensing for the development of clinical early diagnosis.

Keywords: DNA nanomachine; DNA nanoswitch; DNA walker; Trilpex DNA nanoswitch.