Photodegradation enhances the toxic effect of anthracene on skin

Molly Brzezinski; Leisha Martin; Kayla Simpson; Kaijun Lu; Nin Gan; Chi Huang; Kaitlin Garcia; Zhanfei Liu; Wei Xu

doi:10.1016/j.jhazmat.2024.134386

Photodegradation enhances the toxic effect of anthracene on skin

J Hazard Mater. 2024 Apr 22:471:134386. doi: 10.1016/j.jhazmat.2024.134386. Online ahead of print.

Authors

Molly Brzezinski¹, Leisha Martin¹, Kayla Simpson¹, Kaijun Lu², Nin Gan¹, Chi Huang¹, Kaitlin Garcia¹, Zhanfei Liu², Wei Xu³

Affiliations

¹ Department of Life Sciences, College of Science, Texas A&M University - Corpus Christi, Corpus Christi, TX, USA.
² University of Texas at Austin Marine Science Institute 750 Channel View Drive Port Aransas, TX 78373, USA.
³ Department of Life Sciences, College of Science, Texas A&M University - Corpus Christi, Corpus Christi, TX, USA. Electronic address: wei.xu@tamucc.edu.

PMID: 38663297
DOI: 10.1016/j.jhazmat.2024.134386

Abstract

Anthracene, a polycyclic aromatic hydrocarbon (PAH), is a widespread environmental pollutant that poses potential risks to human health. Exposure to anthracene can result in various adverse health effects, including skin-related disorders. Photo exposure sufficiently removes the anthracene from the environment but also generates more degradation products which can be more toxic. The goal of this study was to assess the change in anthracene dermotoxicity caused by photodegradation and understand the mechanism of this change. In the present study, over 99.99% of anthracene was degraded within 24 h of sunlight exposure, while producing many intermediate products including 9,10-anthraquinone and phthalic acid. The anthracene products with different durations of photo exposure were applied to 2D and 3D human keratinocyte cultures. Although the non-degraded anthracene significantly delayed the cell migration, the cell viability and differentiation decreased dramatically in the presence of the photodegraded anthracene. Anthracene photodegradation products also altered the expression patterns of a number of inflammation-related genes in comparison to the control cells. Among these genes, il1a, il1b, il8, cxcl2, s100a9, and mmp1 were upregulated whereas the tlr4 and mmp3 were downregulated by the photodegraded anthracene. Topical deliveries of the photodegraded and non-degraded anthracene to the dorsal skin of hairless mice showed more toxic effects by the photodegraded anthracene. The 4-hour photodegradation products of anthracene thickened the epidermal layer, increased the dermal cellularity, and induced the upregulation of inflammatory markers, il1a, il1b, s100a9, and mmp1. In addition, it also prevented the production of a gap junction protein, Connexin-43. All the evidence suggested that photodegradation enhanced the toxicities of anthracene to the skin. The 4-hour photodegradation products of anthracene led to clinical signs similar to acute inflammatory skin diseases, such as atopic and contact dermatitis, eczema, and psoriasis. Therefore, the potential risk of skin irritation by anthracene should be also considered when an individual is exposed to PAHs, especially in environments with strong sunlight.

Keywords: 9,10-anthraquinone; Anthracene; Keratinocytes; Photodegradation; Skin irritation.