Comprehensive untargeted lipidomics study of black morel (Morchella sextelata) at different growth stages

Guangbo Xie; Maoyuan Chen; Yanran Yang; Yu Xie; Kejun Deng; Liyuan Xie

doi:10.1016/j.foodchem.2024.139431

Comprehensive untargeted lipidomics study of black morel (Morchella sextelata) at different growth stages

Food Chem. 2024 Apr 22:451:139431. doi: 10.1016/j.foodchem.2024.139431. Online ahead of print.

Authors

Guangbo Xie¹, Maoyuan Chen², Yanran Yang², Yu Xie², Kejun Deng², Liyuan Xie³

Affiliations

¹ School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, PR China; Innovation Center of Electronic Information & Traditional Chinese Medicine, University of Electronic Science and Technology of China, Chengdu 610054, PR China. Electronic address: gbxie@uestc.edu.cn.
² School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, PR China.
³ Sichuan Institute of Edible Fungi, Sichuan Academy of Agricultural Sciences, Chengdu 610066, PR China.

PMID: 38663248
DOI: 10.1016/j.foodchem.2024.139431

Abstract

The black morel (Morchella sextelata) is a valuable edible and medicinal mushroom appreciated worldwide. Here, lipidomic profiles and lipid dynamic changes during the growth of M. sexletata were analyzed using ultra-performance liquid chromatography coupled with mass spectrometry. 203 lipid molecules, including four categories and fourteen subclasses, were identified in mature fruiting bodies, with triacylglycerol being the most abundant (37.00 %). Fatty acid composition analysis revealed that linoleic acid was the major fatty acid among the free fatty acids, glycerolipids and glycerophospholipids. The relative concentration of lipids in M. sextelata changed significantly during its growth, from which 12 and 29 differential lipid molecules were screened out, respectively. Pathway analysis based on these differential lipids showed that glycerophospholipid metabolism was the major pathway involved in the growth of M. sextelata. Our study provides a comprehensive understanding of the lipids in M. sextelata and will facilitate the development and utilization of M. sextelata.

Keywords: Black morel; Growth stage; Lipid dynamic changes; Lipidomics; Morchella sextelata; UPLC-MS.