Soybean meal peptide Gly-Thr-Tyr-Trp could protect mice from acute alcoholic liver damage: A study of protein-protein interaction and proteomic analysis

Siwen Lyu; Zhuanzhang Cai; Qi Yang; Jingbo Liu; Yiding Yu; Fengguang Pan; Ting Zhang

doi:10.1016/j.foodchem.2024.139337

Soybean meal peptide Gly-Thr-Tyr-Trp could protect mice from acute alcoholic liver damage: A study of protein-protein interaction and proteomic analysis

Food Chem. 2024 Apr 15:451:139337. doi: 10.1016/j.foodchem.2024.139337. Online ahead of print.

Authors

Siwen Lyu¹, Zhuanzhang Cai¹, Qi Yang¹, Jingbo Liu¹, Yiding Yu¹, Fengguang Pan², Ting Zhang³

Affiliations

¹ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China.
² Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: panfg@jlu.edu.cn.
³ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: tingzhang@jlu.edu.cn.

PMID: 38663243
DOI: 10.1016/j.foodchem.2024.139337

Abstract

Alcoholic liver disease (ALD) is a serious health threat. Soybean meal peptide (SMP) supplementation may protect against this damage; however, the potential mechanism underlying the specific sequence of SMPs is unclear. Protein-protein interaction and proteomic analyses are effective methods for studying functional ingredients in diseases. This study aimed to investigate the potential mechanism of action of the peptide Gly-Thr-Tyr-Trp (GTYW) on ALD using protein-protein interaction and proteomic analyses. These results demonstrate that GTYW influenced the targets of glutathione metabolism (glutathione-disulfide reductase, glutathione S-transferase pi 1, and glutathione S-transferase mu 2). It also regulated the expression of targets related to energy metabolism and amino acid conversion (trypsin-2, cysteine dioxygenase type-1, and F6SJM7). Amino acid and lipid metabolisms were identified based on Gene Ontology annotation. These results indicate that GTYW might affect alcohol-related liver disease signaling pathways. This study provides evidence of the protective and nutritional benefits of SMPs in ALD treatment.

Keywords: Alcohol; Bioactive peptides; Glutathione metabolism; Network pharmacology; Target interaction.