Comparison between commercial media and TRIS-egg yolk extender in the refrigerated storage of collared peccary semen at 17°C

Romário P Santos; Samara S J Moreira; Luana G P Bezerra; Yasmim C S Cavalcante; Ana G Pereira; Tayná M Matos; Moacir F Oliveira; Alexandre R Silva

doi:10.1016/j.anireprosci.2024.107478

Comparison between commercial media and TRIS-egg yolk extender in the refrigerated storage of collared peccary semen at 17°C

Anim Reprod Sci. 2024 Apr 22:265:107478. doi: 10.1016/j.anireprosci.2024.107478. Online ahead of print.

Authors

Romário P Santos¹, Samara S J Moreira¹, Luana G P Bezerra¹, Yasmim C S Cavalcante¹, Ana G Pereira¹, Tayná M Matos¹, Moacir F Oliveira¹, Alexandre R Silva²

Affiliations

¹ Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido (Federal Rural University of the Semiarid Region) - UFERSA, Mossoró, Rio Grande do Norte, Brazil.
² Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido (Federal Rural University of the Semiarid Region) - UFERSA, Mossoró, Rio Grande do Norte, Brazil. Electronic address: alexrs@ufersa.edu.br.

PMID: 38663148
DOI: 10.1016/j.anireprosci.2024.107478

Abstract

To assist in the conservation of collared peccary, it is important to strengthen semen processing protocols. The aim of this study was to compare the effects of different commercial extenders (BTS; NUTRIXcell+ and PRIMXcell Ultra) and TRIS + egg yolk on the functional and morphological aspects of collared peccary semen stored at 17 °C for 48 hours. Ten ejaculates obtained by electroejaculation were divided into 4 aliquots and diluted in the respective extenders, then stored in a biological incubator at 17 °C for 12, 24, 36, and 48 hours. The samples were evaluated for kinetic parameters, membrane functionality, membrane integrity, mitochondrial activity, morphology, and sperm-binding capacity. At the end of storage (48 h), promising results were found for motility parameters, with TRIS + egg yolk (71.0 ± 4.6%) being more efficient than NUTRIXcell+ (38.9 ± 10.9%) (P < 0.05) and similar to BTS (42.9 ± 11.9%) and PRIMXcell Ultra (46.8 ± 10.8%). The results for membrane integrity and mitochondrial activity were around ∼30-50%, with TRIS being the only extender to preserve both parameters (58.9 ± 5.3 and 59.2 ± 5.6%) for up to 48 hours, respectively (P < 0.05). Finally, the extenders could guarantee 60% membrane functionality and ∼ 60-70% normal sperm morphology, as well as similar binding capacity among the groups. In conclusion, TRIS + egg yolk is effective in preserving the sperm parameters of collared peccary semen at 17 °C for 48 hours, while PRIMXcell Ultra and BTS are viable alternatives for this purpose.

Keywords: BTS; NUTRIXcell; PRIMXcell; Short-term storage.