Two new triterpenoid saponins (oleanolic acid 2β-hydroxyl-3-O-β-D-glucuronopyranoside-6'-O-buthyl ester (1) and oleanolic acid 2β-hydroxyl-3-O-[β-D-glucuronopyranosyl-6'-O-methylester]-28-O-β-D-glucopyranoside (2)) and two new goodyerosides (4-methylenefuran-2(5H)-one (6'-O-vanilloyl)-β-D-glucopyranoside (3), 3-hydroxy-2(5H)-furanone, 4-(6'-O-vanilloyl)-β-D-glucopyranoside (4)), together with seven known compounds (5-11) were isolated from the whole plant of Tournefortia sibirica L. The chemical structures of the compounds were determined by spectroscopic analysis (1D and 2D NMR) and HR-ESI-MS. Compounds 1, 6 and 9 showed significant cytotoxicity towards A549, SK-Hep1 and HeLa cells, with IC50 values ranging from 1.68 ± 0.09 to 6.87 ± 0.13 μM.
Keywords: Tournefortia sibirica L; chemical constituents; cytotoxic activity; goodyerosides; triterpenoid saponins.