Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection

José Manuel Cruz-Rubio; Renate Loeppert; Werner Praznik

doi:10.1007/978-1-0716-3782-1_5

Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection

Methods Mol Biol. 2024:2788:67-79. doi: 10.1007/978-1-0716-3782-1_5.

Authors

José Manuel Cruz-Rubio¹, Renate Loeppert², Werner Praznik²

Affiliations

¹ Plant Functional Genomics Lab, Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
² Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria.

PMID: 38656509
DOI: 10.1007/978-1-0716-3782-1_5

Abstract

Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure.

Keywords: 1-Phenyl-3-methyl-5-pyrazolone; Carbohydrate analysis; Chromatography; Monosaccharide chromophores; Monosaccharide derivatization; PMP; Superficially porous columns.

MeSH terms

Antipyrine / analogs & derivatives
Antipyrine / chemistry
Chromatography, High Pressure Liquid / methods
Chromatography, Reverse-Phase* / methods
Edaravone / chemistry
Monosaccharides* / analysis
Monosaccharides* / chemistry
Spectrophotometry, Ultraviolet / methods

Substances

Monosaccharides
Edaravone
Antipyrine