Histological evaluation of the surgical margins of oral soft tissue incisions using a dual-wavelength diode laser and an Er, Cr:YSGG laser; an ex vivo study

J Appl Oral Sci. 2024 Apr 22:32:e20230419. doi: 10.1590/1678-7757-2023-0419. eCollection 2024.

Abstract

Objective: This study compared a dual-wavelength diode laser and an Er, Cr:YSGG laser in oral soft tissue incisions to determine the most effective and safest laser system at the histopathological level.

Methodology: The (810 and 980 nm) dual-wavelength diode laser was used at 1.5 W and 2.5 W (CW) power settings, and the (2780 nm) Er, Cr:YSGG laser was used at 2.5 W and 3.5 W (PW) power settings. Both laser systems were used to incise the tissues of freshly dissected sheep tongue pieces to obtain the following histopathological criteria: epithelial tissue changes, connective tissue changes, and lateral thermal damage extent by optical microscopy.

Results: The epithelial and connective tissue damage scores were significantly higher in the dual-wavelength diode laser groups than in the Er, Cr:YSGG laser groups (P<0.001), and there was a significant difference between some groups. The extent of lateral thermal damage was also significantly higher in the diode laser groups than in the Er, Cr: YSGG laser groups (P<0.001), and there was a significant difference between groups. Group 2 (2.5 W) of the diode laser was the highest for all three criteria, while group 3 (2.5 W) of the Er, Cr:YSGG laser was the lowest.

Conclusion: The Er, Cr:YSGG laser with an output power of 2.5 W is, histologically, the most effective and safest laser for oral soft tissue incision. The dual-wavelength diode laser causes more damage than the Er, Cr:YSGG laser, but it can be used with a low output power and 1 mm safety distance in excisional biopsy.

Publication types

  • Evaluation Study
  • Comparative Study

MeSH terms

  • Animals
  • Connective Tissue / pathology
  • Epithelium / pathology
  • Laser Therapy / instrumentation
  • Laser Therapy / methods
  • Lasers, Semiconductor* / therapeutic use
  • Lasers, Solid-State* / therapeutic use
  • Margins of Excision*
  • Mouth Mucosa / pathology
  • Mouth Mucosa / surgery
  • Oral Surgical Procedures / methods
  • Reference Values
  • Reproducibility of Results
  • Sheep
  • Statistics, Nonparametric
  • Tongue* / pathology
  • Tongue* / surgery