Characterization of Ralstonia insidiosa C1 isolated from Alpine regions: Capability in polyhydroxyalkanoates degradation and production

Young-Cheol Chang; M Venkateswar Reddy; Hinako Suzuki; Takumi Terayama; Yasuteru Mawatari; Chigusa Seki; Omprakash Sarkar

doi:10.1016/j.jhazmat.2024.134348

Characterization of Ralstonia insidiosa C1 isolated from Alpine regions: Capability in polyhydroxyalkanoates degradation and production

J Hazard Mater. 2024 Apr 18:471:134348. doi: 10.1016/j.jhazmat.2024.134348. Online ahead of print.

Authors

Young-Cheol Chang¹, M Venkateswar Reddy², Hinako Suzuki³, Takumi Terayama⁴, Yasuteru Mawatari⁵, Chigusa Seki⁵, Omprakash Sarkar⁶

Affiliations

¹ Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Hokkaido 050-8585, Japan; Department of Sciences and Informatics, Course of Chemical and Biological Systems, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan. Electronic address: ychang@muroran-it.ac.jp.
² Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
³ Department of Sciences and Informatics, Course of Chemical and Biological Systems, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan.
⁴ Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Hokkaido 050-8585, Japan.
⁵ Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Hokkaido 050-8585, Japan; Department of Sciences and Informatics, Course of Chemical and Biological Systems, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan.
⁶ Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, 971 87 Luleå, Sweden.

PMID: 38653138
DOI: 10.1016/j.jhazmat.2024.134348

Abstract

This study ventures into the exploration of potential poly-3-hydroxybutyrate (PHB) degradation in alpine environments. PHB-degrading bacteria were identified in both campus soil, representing a residential area, and Mt. Kurodake soil, an alpine region in Hokkaido, Japan. Next-generation sequencing analysis indicated that the campus soil exhibited higher microbial diversity, while Ralstonia insidiosa C1, isolated from Mt. Kurodake soil, displayed the highest proficiency in PHB degradation. R. insidiosa C1 efficiently degraded up to 3% (w/v) of PHB and various films composed of other biopolymers at 14 °C. This bacterium synthesized homopolymers using substrates such as 3-hydroxybutyric acid, sugars, and acetic acid, while also produced copolymers using a mixture of fatty acids. The analysis results confirmed that the biopolymer synthesized by strain C1 using glucose was PHB, with physical properties comparable to commercial products. The unique capabilities of R. insidiosa C1, encompassing both the production and degradation of bioplastics, highlight its potential to establish a novel material circulation model.

Keywords: Mulch films; Next-generation sequencing; Nuclear magnetic resonance; P(3HB-co-3HV); PHB film; PHBH.