Extraction of Soluble Proteins from Leaves

Abstract

Protein biochemistry can provide valuable answers to better understand plant performance and responses to the surrounding environment. In this chapter, we describe the process of extracting proteins from plant leaf samples. We highlight the key aspects to take into consideration to preserve protein integrity, from sample collection to extraction and preparation or storage for subsequent analysis of protein abundance and/or enzymatic activities.

Keywords: Calvin-Benson-Bassham cycle; Chloroplast proteins; Enzymatic activity; Photosynthetic metabolism; Protein biochemistry; Protein integrity; Protein quantification; Rubisco; Rubisco activase.