Imaging Spatial and Temporal Variation in Photosynthesis Using Chlorophyll Fluorescence

Tracy Lawson; Silvere Vialet-Chabrand

doi:10.1007/978-1-0716-3790-6_15

Imaging Spatial and Temporal Variation in Photosynthesis Using Chlorophyll Fluorescence

Methods Mol Biol. 2024:2790:293-316. doi: 10.1007/978-1-0716-3790-6_15.

Authors

Tracy Lawson¹, Silvere Vialet-Chabrand²

Affiliations

¹ School of Life Sciences, University of Essex, Colchester, UK. tlawson@essex.ac.uk.
² Horticulture and Product Physiology, Department of Plant Sciences, Wageningen University & Research, Wageningen, The Netherlands.

PMID: 38649577
DOI: 10.1007/978-1-0716-3790-6_15

Abstract

Chlorophyll fluorescence imaging provides a noninvasive rapid screen to assess the physiological status of a number of leaves or plants simultaneously. Although there are no standard protocols for chlorophyll fluorescence imaging, here we provide an example of routines for some of the typical measurements.

Keywords: Chlorophyll fluorescence; Imaging; Non-photochemical quenching; Phenotypic screening; Photochemical quenching; Photosynthetic efficiency; Plant stress; Spatial and temporal resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorophyll* / metabolism
Fluorescence
Optical Imaging / methods
Photosynthesis*
Plant Leaves* / metabolism

Substances

Chlorophyll