Measurement of Algal Photosynthesis Using a Clark-Type O2 Electrode

Steven J Burgess; Chandra Davies

doi:10.1007/978-1-0716-3790-6_7

Measurement of Algal Photosynthesis Using a Clark-Type O₂ Electrode

Methods Mol Biol. 2024:2790:121-132. doi: 10.1007/978-1-0716-3790-6_7.

Authors

Steven J Burgess¹, Chandra Davies²

Affiliations

¹ Department of Plant Biology and Carl R Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL, USA. sjb287@illinois.edu.
² Department of Plant Biology and Carl R Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL, USA.

PMID: 38649569
DOI: 10.1007/978-1-0716-3790-6_7

Abstract

The Clark-type electrode can be used to assess the rates of photosynthesis by detecting changes in O₂ concentration in a culture. This chapter describes a method for a liquid phase measurement of light and dissolved inorganic carbon-dependent photosynthesis using the model green alga Chlamydomonas reinhardtii. The technique can be used to evaluate the presence or efficiency of carbon-concentrating mechanisms.

Keywords: Algae; Carbon-concentrating mechanism; Chlamydomonas reinhardtii; Chlorophyll concentration; Clark-type electrode; Light curve; Oxygen electrode; Photosynthesis; Photosynthetic rate constants.

MeSH terms

Carbon / chemistry
Carbon / metabolism
Chlamydomonas reinhardtii* / metabolism
Electrodes*
Light
Oxygen* / metabolism
Photosynthesis*

Substances

Oxygen
Carbon