Transcription regulation strategies in methylotrophs: progress and challenges

Xiaohan Huang; Qiaoqiao Song; Shuqi Guo; Qiang Fei

doi:10.1186/s40643-022-00614-3

Transcription regulation strategies in methylotrophs: progress and challenges

Bioresour Bioprocess. 2022 Dec 12;9(1):126. doi: 10.1186/s40643-022-00614-3.

Authors

Xiaohan Huang¹, Qiaoqiao Song¹, Shuqi Guo¹, Qiang Fei^{2

3}

Affiliations

¹ School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
² School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, China. feiqiang@xjtu.edu.cn.
³ Shaanxi Key Laboratory of Energy Chemical Process Intensification, Xi'an Jiaotong University, Xi'an, 710049, China. feiqiang@xjtu.edu.cn.

Abstract

As a promising industrial microorganism, methylotroph is capable of using methane or methanol as the sole carbon source natively, which has been utilized in the biosynthesis of various bioproducts. However, the relatively low efficiency of carbon conversion has become a limiting factor throughout the development of methanotrophic cell factories due to the unclear genetic background. To better highlight their advantages in methane or methanol-based biomanufacturing, some metabolic engineering strategies, including upstream transcription regulation projects, are being popularized in methylotrophs. In this review, several strategies of transcription regulations applied in methylotrophs are summarized and their applications are discussed and prospected.

Keywords: C1 bioconversion; CRISPR; Methylotrophs; Promoter; Transcription factor; Transcription regulation.

Publication types

Review

Abstract

Publication types

Grants and funding