The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis

Julia Załęcka; Zuzanna Zielińska; Łukasz Ołdak; Agata Sakowicz; Grzegorz Mańka; Mariusz Kiecka; Robert Spaczyński; Piotr Piekarski; Beata Banaszewska; Artur Jakimiuk; Tadeusz Issat; Jakub Młodawski; Maria Szubert; Piotr Sieroszewski; Grzegorz Raba; Kamil Szczupak; Tomasz Kluz; Marek Kluza; Piotr Pierzyński; Michał Ciebiera; Cezary Wojtyła; Michał Lipa; Damian Warzecha; Mirosław Wielgoś; Krzysztof Cendrowski; Ewa Gorodkiewicz; Piotr Laudański

doi:10.1016/j.advms.2024.04.004

The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis

Adv Med Sci. 2024 Apr 18;69(2):224-230. doi: 10.1016/j.advms.2024.04.004. Online ahead of print.

Authors

Julia Załęcka¹, Zuzanna Zielińska², Łukasz Ołdak³, Agata Sakowicz⁴, Grzegorz Mańka⁵, Mariusz Kiecka⁵, Robert Spaczyński⁶, Piotr Piekarski⁷, Beata Banaszewska⁸, Artur Jakimiuk⁹, Tadeusz Issat¹⁰, Jakub Młodawski¹¹, Maria Szubert¹², Piotr Sieroszewski¹³, Grzegorz Raba¹⁴, Kamil Szczupak¹⁴, Tomasz Kluz¹⁵, Marek Kluza¹⁵, Piotr Pierzyński¹⁶, Michał Ciebiera¹⁷, Cezary Wojtyła¹⁸, Michał Lipa¹⁹, Damian Warzecha²⁰, Mirosław Wielgoś²¹, Krzysztof Cendrowski¹, Ewa Gorodkiewicz³, Piotr Laudański²²

Affiliations

¹ Chair and Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Poland.
² Bioanalysis Laboratory, Doctoral School of Exact and Natural Science, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok, Poland.
³ Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok, Poland.
⁴ Department of Medical Biotechnology, Medical University of Lodz, Lodz, Poland.
⁵ Angelius Hospital, Katowice, Poland.
⁶ Center for Gynecology, Obstetrics and Infertility Treatment Pastelova, Poznan, Poland; Collegium Medicum, Institute of Health Sciences, University of Zielona Gora, Poland.
⁷ Gynecological Obstetric Clinical Hospital of Poznan University of Medical Sciences, Minimally Invasive Gynecological Surgery, Poznan, Poland.
⁸ Chair and Department of Laboratory Diagnostics Poznan University of Medical Sciences, Poland.
⁹ Department of Reproductive Health, Institute of Mother and Child in Warsaw, Warsaw, Poland; Department of Gynecology, Oncological Gynecology and Reproduction, National Medical Institute of the Ministry of the Interior and Administration, Warsaw, Poland.
¹⁰ Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, Warsaw, Poland.
¹¹ Collegium Medicum Jan Kochanowski University in Kielce, Poland; Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, Kielce, Poland.
¹² Department of Gynecology and Obstetrics, Medical University of Lodz, Lodz, Poland; Department of Surgical Gynecology and Oncology, Medical University of Lodz, Lodz, Poland.
¹³ Department of Gynecology and Obstetrics, Medical University of Lodz, Lodz, Poland; Department of Fetal Medicine and Gynecology, Medical University of Lodz, Lodz, Poland.
¹⁴ Clinic of Obstetrics and Gynecology in Przemysl, Przemysl, Poland; Department of Obstetrics and Gynecology, University of Rzeszow, Rzeszow, Poland.
¹⁵ Department of Gynecology, Gynecology Oncology and Obstetrics, Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland.
¹⁶ OVIklinika Infertility Center, Warsaw, Poland.
¹⁷ Second Department of Obstetrics and Gynecology, Centre of Postrgraduate Medical Education, Warsaw, Poland; Warsaw Institute of Women's Health, Warsaw, Poland.
¹⁸ OVIklinika Infertility Center, Warsaw, Poland; Women's Health Research Institute, Calisia University, Kalisz, Poland.
¹⁹ Department of Obstetrics and Perinatology, National Medical Institute of the Ministry of the Interior and Administration, Warsaw, Poland.
²⁰ OVIklinika Infertility Center, Warsaw, Poland; City South Hospital Warsaw, Warsaw, Poland; Faculty of Medicine, University of Warsaw, Warsaw, Poland.
²¹ Department of Obstetrics and Perinatology, National Medical Institute of the Ministry of the Interior and Administration, Warsaw, Poland; Premium Medical Clinic, Warsaw, Poland; Medical Faculty, Lazarski University, Warsaw, Poland.
²² Chair and Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Poland; OVIklinika Infertility Center, Warsaw, Poland; Women's Health Research Institute, Calisia University, Kalisz, Poland. Electronic address: piotr.laudanski@wum.edu.pl.

PMID: 38642611
DOI: 10.1016/j.advms.2024.04.004

Abstract

Purpose: Endometriosis is a common disease with a complex pathomechanism and atypical symptoms, often leading to delayed diagnosis. Currently, the sole method for confirming the presence of the disease is through laparoscopy and histopathological examination of collected tissue. However, this invasive procedure carries potential risk and complications, necessitating the exploration of non-surgical diagnostic methods for endometriosis. This study aims to analyze peritoneal fluid and plasma samples for the expression of cathepsin L and cathepsin S to identify potential biomarkers for non-invasive diagnostic approaches to endometriosis.

Material and methods: In this cross-sectional study, plasma and peritoneal fluid samples were obtained during laparoscopy from 63 patients diagnosed with chronic pelvic pain or infertility. The study group consisted of women with confirmed endometriosis. The concentrations of cathepsins L and S were determined using an SPRi biosensor.

Results: The study did not reveal significant differences in the concentrations of cathepsin L and cathepsin S between the control group and the study group, both in peritoneal fluid and plasma.

Conclusions: Based on the results of this study, it appears that cathepsins L and S are not suitable candidates as biomarkers for endometriosis.

Keywords: Cathepsin L; Cathepsin S; Endometriosis; Peritoneal fluid; Plasma.