Population structure and genetic diversity of Polish Arabian horses based on pedigree data

W Klecel; M Kloch; M Wojciechowska; M Gajewska; E Martyniuk

doi:10.1016/j.animal.2024.101148

Population structure and genetic diversity of Polish Arabian horses based on pedigree data

Animal. 2024 Mar 27;18(5):101148. doi: 10.1016/j.animal.2024.101148. Online ahead of print.

Authors

W Klecel¹, M Kloch², M Wojciechowska², M Gajewska², E Martyniuk²

Affiliations

¹ Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-787 Warsaw, Poland. Electronic address: weronika_klecel@sggw.edu.pl.
² Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-787 Warsaw, Poland.

PMID: 38642411
DOI: 10.1016/j.animal.2024.101148

Abstract

With a history tracing back to at least the 18th century and a substantial global influence on various breeds, Polish Arabian horse population is of paramount importance for both breeders and conservationists. However, its genetic makeup and the population dynamics are still not well understood. This study presents an analysis of the modern Polish Arabian horse population using pedigree data, focusing on the breed's genetic diversity and population structure. Our analysis encompassed 1 498 individuals defined as the reference population (RP) and their 11 065 ancestors, which resulted in the dataset of 12 254 individuals (total population). We traced their genealogy to assess inbreeding coefficients (F), founder effects, and genetic variability measures such as the effective number of founders (f_e), ancestors (f_a), or founder genome equivalents (f_ge). The results indicated a good pedigree quality with an average of 28.1 maximum traced generations, revealing high pedigree completeness for initial generations with a decline beyond the seventh generation. The genetic diversity parameters showed a considerable bottleneck effect, with an effective number of founders at 73, which reflects a substantial loss of genetic diversity over time. Despite the vast total number of founders (852), only a few have had a lasting impact on the current population, signaling the need for revised breeding strategies to maintain diversity. The study identified a slight but consistent rise in inbreeding over the last century, with a marginal recent decline, and a significant difference in the contribution of various founders. The average F was 5.8%, with 99.6% of the reference population being inbred. The analysis of effective population size (N_e) highlighted potential risks for genetic diversity, urging for revision of breeding goals to consider a wider array of founder lineages. The study indicated that stallions belonging to RP can be attributed to 15 distinct sirelines, whereas mares to 45 unique damlines, more than considered in the current breeding program (8 and 15, respectively). Conclusively, the study underlines the need for ongoing monitoring and strategic breeding to maintain and enhance the genetic diversity of Polish Arabians, considering the breed's historical significance and contemporary genetic challenges.

Keywords: Bottleneck effect; Equine; Generation interval; Inbreeding; Population founders.