Pt/NiFe-LDH hybrids for quantification and qualification of polyphenols

Chunmeng Ding; Yuexing Zhu; Zhiyuan Huo; Shouzhi Yang; Yan Zhou; Ayizekeranmu Yiming; Wei Chen; Shanrong Liu; Kun Qian; Lin Huang

doi:10.1016/j.mtbio.2024.101047

Pt/NiFe-LDH hybrids for quantification and qualification of polyphenols

Mater Today Bio. 2024 Apr 3:26:101047. doi: 10.1016/j.mtbio.2024.101047. eCollection 2024 Jun.

Authors

Chunmeng Ding^{1

2}, Yuexing Zhu³, Zhiyuan Huo¹, Shouzhi Yang², Yan Zhou¹, Ayizekeranmu Yiming², Wei Chen², Shanrong Liu³, Kun Qian², Lin Huang^{1

4}

Affiliations

¹ Department of Clinical Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
² State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
³ Second Military Medical University, Changhai Hospital, Department of Lab Diagnostics, Shanghai, 200433, P. R. China.
⁴ Institute of Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, P. R. China.

Abstract

Polyphenols with antioxidant properties are of significant interest in medical and pharmaceutical applications. Given the diverse range of activities of polyphenols in vivo, accurate detection of these compounds plays a crucial role in nutritional surveillance and pharmaceutical development. Yet, the efficient quantitation of polyphenol contents and qualification of monomer compositions present a notable challenge when studying polyphenol bioavailability. In this study, platinum-modified nickel-iron layered double hydroxide (Pt/NiFe-LDH hybrids) were designed to mimic peroxidases for colorimetric analysis and act as enhanced matrices for laser desorption/ionization mass spectrometry (LDI MS) to quantify and qualify polyphenols. The hybrids exhibited an enzymatic activity of 33.472 U/mg for colorimetric assays, facilitating the rapid and direct quantitation of total tea polyphenols within approximately 1 min. Additionally, the heterogeneous structure and exposed hydroxyl groups on the hybrid surface contributed to photoelectric enhancement and in-situ enrichment of polyphenols in LDI MS. This study introduces an innovative approach to detect polyphenols using advanced materials, potentially inspiring the future development and applications of other photoactive nanomaterials.

Keywords: Laser desorption/ionization mass spectrometry; Nanozyme; Polyphenol; Pt/NiFe-LDH hybrid; Qualification; Quantification.