Heat shock protein 71 restricts mutation of porcine reproductive and respiratory syndrome virus nsp2 in vitro

Fengyu Xie; Lei Kang; Mengli Chen; Tong Zhang; Zongjie Li; Donghua Shao; Beibei Li; Jianchao Wei; Yafeng Qiu; Manxiang Li; Zhiyong Ma; Ke Liu

doi:10.1016/j.cimid.2024.102179

Heat shock protein 71 restricts mutation of porcine reproductive and respiratory syndrome virus nsp2 in vitro

Comp Immunol Microbiol Infect Dis. 2024 Apr 15:109:102179. doi: 10.1016/j.cimid.2024.102179. Online ahead of print.

Authors

Fengyu Xie¹, Lei Kang², Mengli Chen³, Tong Zhang², Zongjie Li², Donghua Shao², Beibei Li², Jianchao Wei², Yafeng Qiu², Manxiang Li⁴, Zhiyong Ma⁵, Ke Liu⁶

Affiliations

¹ College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai 200241, China.
² Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai 200241, China.
³ Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai 200241, China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
⁴ Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 518, Ziyue Road, Shanghai 200241, China. Electronic address: manxiangl@163.com.
⁵ College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China. Electronic address: Zhiyongma@shvri.ac.cn.
⁶ College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China. Electronic address: liuke@shvri.ac.cn.

PMID: 38636297
DOI: 10.1016/j.cimid.2024.102179

Abstract

porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection, is an important swine infectious disease that causes substantial losses worldwide each year. PRRSV is a positive-sense single-stranded RNA virus that is highly susceptible to mutation and recombination, making vaccine and drug research for the disease extremely difficult. In this study, the binding of PRRSV nsp2 to HSP71 protein was detected by using the IP/MS technique. And the inhibitory effect of HSP71 on nsp2 antagonistic activity was validated by measuring NF-kB luciferase reporter. According to stress from inhibitory effects, the amino acid variation profile of PRRSV nsp2 under HSP71 stress was further analyzed using second-generation sequencing. Surprisingly, the results indicated that HSP71 pressure limits the random mutations of PRRSV nsp2 and maintains the dominant PRRSV strain within the population. Mutant strain showed weaker antagonistic activity and replication capability in cell. These results imply the binding of HSP71 with PRRSV nsp2 may lead to maintain the stability of highly virulent strains of PRRSV.

Keywords: Heat shock protein 71; Mutation; Porcine reproductive and respiratory syndrome virus nsp2.