Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish

Jing Huang; Chao Sun; Zhuo Huang; Yong Zhu; Shi Xi Chen

doi:10.1016/j.jsbmb.2024.106521

Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish

J Steroid Biochem Mol Biol. 2024 Apr 16:241:106521. doi: 10.1016/j.jsbmb.2024.106521. Online ahead of print.

Authors

Jing Huang¹, Chao Sun¹, Zhuo Huang¹, Yong Zhu², Shi Xi Chen³

Affiliations

¹ State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102, China.
² State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102, China; Department of Biology, East Carolina University, 101 E. 10th Street, Greenville, NC 27858, USA.
³ State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen, Fujian 361102, China. Electronic address: chenshixi@xmu.edu.cn.

PMID: 38631601
DOI: 10.1016/j.jsbmb.2024.106521

Abstract

Increased cortisol levels in the preovulatory follicular fluid suggests a role of glucocorticoid in human ovulation. However, the mechanisms through which cortisol regulates the ovulatory process remain poorly understood. In this study, we examined the upregulation of f5 mRNA by glucocorticoid and its receptor (Gr) in the preovulatory follicles of zebrafish. Our findings demonstrate a significant increase in 11β-hydroxysteroid dehydrogenase type 2 (hsd11b2), a cortisol response gene, in preovulatory follicles. Additionally, hydrocortisone exerts a dose- and time-dependent upregulation of f5 mRNA in these follicles. Importantly, this stimulatory effect is Gr-dependent, as it was completely abolished in gr^-/- mutants. Furthermore, site-directed mutagenesis identified a glucocorticoid response element (GRE) in the promoter of zebrafish f5. Interestingly, successive incubation of hydrocortisone and the native ovulation-inducing steroid, progestin (17α,20β-dihydroxy-4-pregnen-3-one, DHP), further enhanced f5 expression in preovulatory follicles. Overall, our results indicate that the dramatic increase of f5 expression in preovulatory follicles is partially attributable to the regulation of glucocorticoid and Gr.

Keywords: Coagulation factor; Glucocorticoid; Glucocorticoid receptor; Ovarian follicles.