Characteristic and mechanism of biological nitrogen and phosphorus removal facilitated by biogenic manganese oxides (BioMnOx) at various concentrations of Mn(II)

Ying Cao; Sicheng Shao; Zhiqing Ye; Chunxiao Wang; Dandan Pan; Xiangwei Wu

doi:10.1016/j.envres.2024.118943

Characteristic and mechanism of biological nitrogen and phosphorus removal facilitated by biogenic manganese oxides (BioMnOx) at various concentrations of Mn(II)

Environ Res. 2024 Apr 16;252(Pt 2):118943. doi: 10.1016/j.envres.2024.118943. Online ahead of print.

Authors

Ying Cao¹, Sicheng Shao¹, Zhiqing Ye¹, Chunxiao Wang¹, Dandan Pan¹, Xiangwei Wu²

Affiliations

¹ College of Resources and Environment, Anhui Agricultural University, Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-Food Quality Safety, Hefei, 230036, China.
² College of Resources and Environment, Anhui Agricultural University, Anhui Provincial Key Laboratory of Hazardous Factors and Risk Control of Agri-Food Quality Safety, Hefei, 230036, China. Electronic address: wxwahau@ahau.edu.cn.

PMID: 38631471
DOI: 10.1016/j.envres.2024.118943

Abstract

Biogenic manganese oxides (BioMnOx) have attracted considerable attention as active oxidants, adsorbents, and catalysts. However, characteristics and mechanisms of nitrification-denitrification in biological redox reactions mediated by different concentrations of BioMnOx are still unclear. Fate of nutrients (e.g., NH₄⁺-N, TP, NO₃^--N) and COD were investigated through different concentrations of BioMnOx produced by Mn(II) in the moving bed biofilm reactor (MBBR). 34% and 89.2%, 37.8% and 89.8%, 57.3% and 88.9%, and 62.1% and 90.4% of TN and COD by MBBR were synchronously removed in four phases, respectively. The result suggested that Mn(II) significantly improved the performance of simultaneous nitrification and denitrification (SND) and TP removal based on manganese (Mn) redox cycling. Characteristics of glutathione peroxidase (GSH-Px), reactive oxygen species (ROS), and electron transfer system activity (ETSA) were discussed, demonstrating that ROS accumulation reduced the ETSA and GSH-Px activities when Mn(II) concentration increased. Extracellular polymeric substance (EPS) function and metabolic pathway of Mn(II) were explored. Furthermore, effect of cellular components on denitrification was evaluated including BioMnOx performances, indicating that Mn(II) promoted the non-enzymatic action of cell fragments. Finally, mechanism of nitrification and denitrification, denitrifying phosphorus and Mn removal was further elucidated through X-ray photoelectron spectroscopy (XPS), high throughput sequencing, and fourier transform infrared reflection (FTIR). This results can bringing new vision for controlling nutrient pollution in redox process of Mn(II).

Keywords: Biogenic manganese oxides (BioMnOx); Denitrification; Microbial community; Moving bed biofilm reactor (MBBR); Nutrients.